Universität zu Köln

Cagnetta, Roberta, Frese, Christian K., Shigeoka, Toshiaki, Krijgsveld, Jeroen and Holt, Christine E. (2018). Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron, 99 (1). S. 29 - 51. CAMBRIDGE: CELL PRESS. ISSN 1097-4199

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Abstract

Axonal protein synthesis and degradation are rapidly regulated by extrinsic signals during neural wiring, but the full landscape of proteomic changes remains unknown due to limitations in axon sampling and sensitivity. By combining pulsed stable isotope labeling of amino acids in cell culture with single-pot solid-phase-enhanced sample preparation, we characterized the nascent proteome of isolated retinal axons on an unparalleled rapid timescale (5 min). Our analysis detects 350 basally translated axonal proteins on average, including several linked to neurological disease. Axons stimulated by different cues (Netrin-1, BDNF, Sema3A) show distinct signatures with more than 100 different nascent protein species up-or downregulated within the first 5 min followed by further dynamic remodeling. Switching repulsion to attraction triggers opposite regulation of a subset of common nascent proteins. Our findings thus reveal the rapid remodeling of the axonal proteomic landscape by extrinsic cues and uncover a logic underlying attraction versus repulsion.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cagnetta, Roberta UNSPECIFIED UNSPECIFIED UNSPECIFIED Frese, Christian K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Shigeoka, Toshiaki UNSPECIFIED UNSPECIFIED UNSPECIFIED Krijgsveld, Jeroen UNSPECIFIED UNSPECIFIED UNSPECIFIED Holt, Christine E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-179804
DOI:	10.1016/j.neuron.2018.06.004
Journal or Publication Title:	Neuron
Volume:	99
Number:	1
Page Range:	S. 29 - 51
Date:	2018
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1097-4199
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESSENGER-RNA TRANSLATION; RETINAL GROWTH CONES; CELL-ADHESION MOLECULE; LOCAL TRANSLATION; IN-VIVO; SYNAPSE FORMATION; LAMININ RECEPTOR; NEURONAL GROWTH; BINDING-PROTEIN; GANGLION-CELLS Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/17980