Kopp, Sascha, Krueger, Marcus, Feldmann, Stefan, Oltmann, Hergen, Schuette, Andreas, Schmitz, Burkhard, Bauer, Johann, Schulz, Herbert, Saar, Kathrin ORCID: 0000-0002-4483-1557, Huebner, Norbert, Wehland, Markus ORCID: 0000-0002-8160-859X, Nassef, Mohamed Zakaria, Melnik, Daniela, Meltendorf, Stefan, Infanger, Manfred and Grimm, Daniela (2018). Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission - The THYROID Project. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Human follicular thyroid cancer cells (FTC-133) were sent to space via a sounding rocket during the TEXUS-53 mission to determine the impact of short-term microgravity on these cells. To enable cell culture and fixation in real microgravity, an automated experiment container (EC) was constructed. In order to ensure safe cell culture, cell-chambers consisting of polycarbonate (PC) material were used. They were highly biocompatible as proved by measuring cell survival using Annexin V flow cytometry. In the follow-up experiment, FTC-133 cells were sent to space via a sounding rocket and were fixed before and after the microgravity (mu g) phase with RNAlater. In addition, cells were tested for reactions on hypergravity (hyper-g) as much as 18 g to determine whether worst case acceleration during launch can have an influence on the cells. We investigated genes belonging to biological processes such as cytoskeleton, cell adhesion, tumor growth, angiogenesis and apoptosis. Pathway analyses revealed central functions of VEGFA and EGF. EGF upregulates aspartate beta-hydroxylase (ASPH) which is influencing CASP3. Hyper-g induced a significant up-regulation of TUBB1, VIM, RDX, CAV1, VEGFA and BCL2. FTC-133 cells grown in an automated EC exposed to mu g revealed moderate gene expression changes indicating their survival in orbit.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kopp, SaschaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Feldmann, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oltmann, HergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schuette, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmitz, BurkhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, JohannUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, HerbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saar, KathrinUNSPECIFIEDorcid.org/0000-0002-4483-1557UNSPECIFIED
Huebner, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wehland, MarkusUNSPECIFIEDorcid.org/0000-0002-8160-859XUNSPECIFIED
Nassef, Mohamed ZakariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Melnik, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meltendorf, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Infanger, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grimm, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-179850
DOI: 10.1038/s41598-018-28695-1
Journal or Publication Title: Sci Rep
Volume: 8
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
DIFFERENTIAL GENE-EXPRESSION; PARABOLIC FLIGHT MANEUVERS; RANDOM POSITIONING MACHINE; ALTERED GRAVITY CONDITIONS; SIMULATED MICROGRAVITY; 3-DIMENSIONAL GROWTH; EXTRACELLULAR-MATRIX; SPHEROID FORMATION; HUMAN CHONDROCYTES; ENDOTHELIAL-CELLSMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/17985

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