Benton, Matthew A. ORCID: 0000-0001-7953-0765 (2018). A revised understanding of Tribolium morphogenesis further reconciles short and long germ development. PLoS. Biol., 16 (7). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1545-7885

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Abstract

In Drosophila melanogaster, the germband forms directly on the egg surface and solely consists of embryonic tissue. In contrast, most insect embryos undergo a complicated set of tissue rearrangements to generate a condensed, multilayered germband. The ventral side of the germband is embryonic, while the dorsal side is thought to be an extraembryonic tissue called the amnion. While this tissue organisation has been accepted for decades and has been widely reported in insects, its accuracy has not been directly tested in any species. Using live cell tracking and differential cell labelling in the short germ beetle Tribolium castaneum, I show that most of the cells previously thought to be amnion actually give rise to large parts of the embryo. This process occurs via the dorsal-to-ventral flow of cells and contributes to germband extension (GBE). In addition, I show that true 'amnion' cells in Tribolium originate from a small region of the blastoderm. Together, my findings show that development in the short germ embryos of Tribolium and the long germ embryos of Drosophila is more similar than previously proposed. Dorsal-to-ventral cell flow also occurs in Drosophila during GBE, and I argue that the flow is driven by a conserved set of underlying morphogenetic events in both species. Furthermore, the revised Tribolium fate map that I present is far more similar to that of Drosophila than the classic Tribolium fate map. Lastly, my findings show that there is no qualitative difference between the tissue structure of the cellularised blastoderm and the short/intermediate germ germband. As such, the same tissue patterning mechanisms could function continuously throughout the cellularised blastoderm and germband stages, and easily shift between them over evolutionary time.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Benton, Matthew A.UNSPECIFIEDorcid.org/0000-0001-7953-0765UNSPECIFIED
URN: urn:nbn:de:hbz:38-180677
DOI: 10.1371/journal.pbio.2005093
Journal or Publication Title: PLoS. Biol.
Volume: 16
Number: 7
Date: 2018
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1545-7885
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Zoologisches Institut
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CELL-SHAPE CHANGES; PAIR-RULE GENE; ONCOPELTUS-FASCIATUS; CYCLORRHAPHAN FLIES; AXIS ELONGATION; SEGMENTATION; DROSOPHILA; INSECTS; CASTANEUM; EXTENSIONMultiple languages
Biochemistry & Molecular Biology; BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18067

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