Universität zu Köln

Venter, Paul Christiaan, Nitsche, Frank and Arndt, Hartmut ORCID: 0000-0003-2811-3595 (2018). The Hidden Diversity of Flagellated Protists in Soil. Protist, 169 (3). S. 432 - 450. MUNICH: ELSEVIER GMBH. ISSN 1434-4610

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Abstract

Protists are among the most diverse and abundant eukaryotes in soil. However, gaps between described and sequenced protist morphospecies still present a pending problem when surveying environmental samples for known species using molecular methods. The number of sequences in the molecular PR2 database (similar to 130,000) is limited compared to the species richness expected (>1 million protist species) limiting the recovery rate. This is important, since high throughput sequencing (HTS) methods are used to find associative patterns between functional traits, taxa and environmental parameters. We performed HTS to survey soil flagellates in 150 grasslands of central Europe, and tested the recovery rate of ten previously isolated and cultivated cercomonad species, among locally found diversity. We recovered sequences for reference soil flagellate species, but also a great number of their phylogenetically evaluated genetic variants, among rare and dominant taxa with presumably own biogeography. This was recorded among dominant (cercozoans, Sandona), rare (apusozoans) and a large hidden diversity of predominantly aquatic protists in soil (choanoflagellates, bicosoecids) often forming novel clades associated with uncultured environmental sequences. Evaluating the reads, instead of the OTUs that individual reads are usually clustered into, we discovered that much of this hidden diversity may be lost due to clustering. (C) 2018 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Venter, Paul Christiaan UNSPECIFIED UNSPECIFIED UNSPECIFIED Nitsche, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-181999
DOI:	10.1016/j.protis.2018.04.007
Journal or Publication Title:	Protist
Volume:	169
Number:	3
Page Range:	S. 432 - 450
Date:	2018
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1434-4610
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPERATIONAL TAXONOMIC UNITS; HIGHLY DIVERSE; CHOANOFLAGELLATE; PHYLOGENY; COMMUNITY; BIOGEOGRAPHY; GENERA; SPEC.; NOV Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18199