Universität zu Köln

Ahmed, Shahira A. and Karanis, Panagiotis (2018). Comparison of current methods used to detect Cryptosporidium oocysts in stools. Int. J. Hyg. Environ. Health., 221 (5). S. 743 - 764. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 1618-131X

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Abstract

In this review all of the methods that are currently in use for the investigation of Cryptosporidium in stool material are highlighted and critically discussed. It appears that more qualifications and background knowledge in this field regarding the diagnosis of the Cryptosporidiwn parasite is required. Furthermore, there is no standardization for the protocols that are commonly used to either detect oocysts in faeces or to diagnose the Cryptosporidizurt infection. It is therefore necessary to initiate further education and research that will assist in improving the accuracy of the diagnosis of Cryptosporidium oocysts in the faecal micro-cosmos. Where ambient concentrations of oocysts are low in stool material, detection becomes a formidable task. Procedures for ring tests and the standardization of multi-laboratory testing are recommended. It is also necessary to enhance the routine surveillance capacity of cryptosporidiosis and to improve the safety against it, considering the fact that this disease is under diagnosed and under reported. This review is intended to stimulate research that could lead to future improvements and further developments in monitoring the diagnostic methodologies that will assist in harmonizing Cryptosporidium oocysts in stool diagnosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ahmed, Shahira A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-184053
DOI:	10.1016/j.ijheh.2018.04.006
Journal or Publication Title:	Int. J. Hyg. Environ. Health.
Volume:	221
Number:	5
Page Range:	S. 743 - 764
Date:	2018
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	1618-131X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYMERASE-CHAIN-REACTION; IN-SITU HYBRIDIZATION; HUMAN FECAL SPECIMENS; REAL-TIME PCR; HETERODUPLEX MOBILITY ASSAY; DIRECT FLUORESCENT-ANTIBODY; ACID-FAST STAIN; PARVUM OOCYSTS; IMMUNOMAGNETIC SEPARATION; GIARDIA-LAMBLIA Multiple languages Public, Environmental & Occupational Health; Infectious Diseases Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/18405