Urban, Maximilian J., Both, Steffen, Zhou, Chao ORCID: 0000-0003-1185-9964, Kuzyk, Anton ORCID: 0000-0001-8060-6122, Lindfors, Klas ORCID: 0000-0002-6482-5605, Weiss, Thomas and Liu, Na (2018). Gold nanocrystal-mediated sliding of doublet DNA origami filaments. Nat. Commun., 9. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Sliding is one of the fundamental mechanical movements in machinery. In macroscopic systems, double-rack pinion machines employ gears to slide two linear tracks along opposite directions. In microscopic systems, kinesin-5 proteins crosslink and slide apart antiparallel microtubules, promoting spindle bipolarity and elongation during mitosis. Here we demonstrate an artificial nanoscopic analog, in which gold nanocrystals can mediate coordinated sliding of two antiparallel DNA origami filaments powered by DNA fuels. Stepwise and reversible sliding along opposite directions is in situ monitored and confirmed using fluorescence spectroscopy. A theoretical model including different energy transfer mechanisms is developed to understand the observed fluorescence dynamics. We further show that such sliding can also take place in the presence of multiple DNA sidelocks that are introduced to inhibit the relative movements. Our work enriches the toolbox of DNA-based nanomachinery, taking one step further toward the vision of molecular nanofactories.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Urban, Maximilian J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Both, SteffenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, ChaoUNSPECIFIEDorcid.org/0000-0003-1185-9964UNSPECIFIED
Kuzyk, AntonUNSPECIFIEDorcid.org/0000-0001-8060-6122UNSPECIFIED
Lindfors, KlasUNSPECIFIEDorcid.org/0000-0002-6482-5605UNSPECIFIED
Weiss, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liu, NaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-189354
DOI: 10.1038/s41467-018-03882-w
Journal or Publication Title: Nat. Commun.
Volume: 9
Date: 2018
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SELF-ORGANIZATION; MOLECULAR MOTOR; WALKER; MICROTUBULES; PROTEINS; KINETICS; DEVICES; WALKING; MOTION; MOVESMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/18935

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