Universität zu Köln

Muller, Gabriela L., Lara, Maria V., Oitaven, Pablo, Andreo, Carlos S., Maurino, Veronica G. and Drincovich, Maria F. (2018). Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells. Sci Rep, 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Severe droughts are predicted for the twenty-first century, which contrast with the increased demand for plant materials. Thus, to sustain future generations, a great challenge is to improve crop yield and water use efficiency (WUE), which is the carbon gained per water lost. Here, expression of maize NADP-malic enzyme (NADP-ME) in the guard and vascular companion cells of Nicotiana tabacum results in enhanced WUE, earlier flowering and shorter life cycle. Transgenic lines exhibit reduced stomatal aperture than wild-type (WT). Nevertheless, an increased net CO2 fixation rate is observed, which results in less water consumption and more biomass production per water used. Transgenic lines export sugars to the phloem at higher rate than WT, which leads to higher sugars levels in phloem exudates and veins. Leaf quantitative proteomic profiling revealed drastic differences in proteins related to cell cycle, flowering, hormone signaling and carbon metabolism between transgenic lines and WT. We propose that the increased sugar export from leaves in the transgenic lines alleviates sugar negative feedback on photosynthesis and thus, stomatal closure takes place without a penalty in CO2 assimilation rate. This results in improved WUE and accelerated overall life cycle, key traits for plant productivity in the near future world.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Muller, Gabriela L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lara, Maria V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oitaven, Pablo UNSPECIFIED UNSPECIFIED UNSPECIFIED Andreo, Carlos S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Drincovich, Maria F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-192866
DOI:	10.1038/s41598-018-22431-5
Journal or Publication Title:	Sci Rep
Volume:	8
Date:	2018
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NADP-MALIC ENZYME; SUCROSE TRANSPORTER SUT1; COMPREHENSIVE ANALYSIS; STOMATAL MOVEMENT; ACID METABOLISM; FLOWERING TIME; TOMATO FRUIT; C-3 PLANTS; ARABIDOPSIS; PHOTOSYNTHESIS Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/19286