Mayer, Michael ORCID: 0000-0002-7820-1393, Takegami, Shigehiko, Neumeier, Michael, Rink, Simone, Jacobi von Wangelin, Axel, Schulte, Silja, Vollmer, Moritz, Griesbeck, Axel G., Duerkop, Axel ORCID: 0000-0003-3007-6471 and Baeumner, Antje J. (2018). Electrochemiluminescence Bioassays with a Water-Soluble Luminol Derivative Can Outperform Fluorescence Assays. Angew. Chem.-Int. Edit., 57 (2). S. 408 - 412. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3773

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Abstract

The most efficient and commonly used electro-chemiluminescence (ECL) emitters are luminol, [Ru(bpy)(3)](2+), and derivatives thereof. Luminol stands out due to its low excitation potential, but applications are limited by its insolubility under physiological conditions. The water-soluble mcarboxy luminol was synthesized in 15% yield and exhibited high solubility under physiological conditions and afforded a four-fold ECL signal increase (vs. luminol). Entrapment in DNA-tagged liposomes enabled a DNA assay with a detection limit of 3.2 pmol L-1, which is 150 times lower than the corresponding fluorescence approach. This remarkable sensitivity gain and the low excitation potential establish m-carboxy luminol as a superior ECL probe with direct relevance to chemiluminescence and enzymatic bioanalytical approaches.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Mayer, MichaelUNSPECIFIEDorcid.org/0000-0002-7820-1393UNSPECIFIED
Takegami, ShigehikoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neumeier, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rink, SimoneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jacobi von Wangelin, AxelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulte, SiljaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vollmer, MoritzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Griesbeck, Axel G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Duerkop, AxelUNSPECIFIEDorcid.org/0000-0003-3007-6471UNSPECIFIED
Baeumner, Antje J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-199064
DOI: 10.1002/anie.201708630
Journal or Publication Title: Angew. Chem.-Int. Edit.
Volume: 57
Number: 2
Page Range: S. 408 - 412
Date: 2018
Publisher: WILEY-V C H VERLAG GMBH
Place of Publication: WEINHEIM
ISSN: 1521-3773
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
LIPOSOMES; CHEMILUMINESCENCEMultiple languages
Chemistry, MultidisciplinaryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/19906

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