Luetzenberg, Ronald, Solano, Kendrick, Buken, Christoph, Sahana, Jayashree ORCID: 0000-0002-6606-6315, Riwaldt, Stefan, Kopp, Sascha, Krueger, Marcus, Schulz, Herbert, Saar, Kathrin, Huebner, Norbert, Hemmersbach, Ruth, Bauer, Johann, Infanger, Manfred, Grimm, Daniela and Wehland, Markus ORCID: 0000-0002-8160-859X (2018). Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes. Cell. Physiol. Biochem., 47 (4). S. 1729 - 1742. BASEL: KARGER. ISSN 1421-9778

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Abstract

Background/Aims: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes. Methods: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profile which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis. Results: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were significantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecific stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM. Conclusions: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes. (C) 2018 The Author(s) Published by S. Karger AG, Basel

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Luetzenberg, RonaldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Solano, KendrickUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buken, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sahana, JayashreeUNSPECIFIEDorcid.org/0000-0002-6606-6315UNSPECIFIED
Riwaldt, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kopp, SaschaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, HerbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Saar, KathrinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huebner, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hemmersbach, RuthUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, JohannUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Infanger, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grimm, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wehland, MarkusUNSPECIFIEDorcid.org/0000-0002-8160-859XUNSPECIFIED
URN: urn:nbn:de:hbz:38-200171
DOI: 10.1159/000491006
Journal or Publication Title: Cell. Physiol. Biochem.
Volume: 47
Number: 4
Page Range: S. 1729 - 1742
Date: 2018
Publisher: KARGER
Place of Publication: BASEL
ISSN: 1421-9778
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ALTERED GRAVITY CONDITIONS; GENE-EXPRESSION; INTERMEDIATE-FILAMENTS; EXTRACELLULAR-MATRIX; ARTICULAR-CARTILAGE; OSTEOPONTIN; CELLS; CYTOSKELETON; PROTEINS; MICROGRAVITYMultiple languages
Cell Biology; PhysiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/20017

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