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Paulsson, Mats and Wagener, Raimund (2018). Matrilins. In: Methods in Cell Biology, S. 429 - 447. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC. ISBN 978-0-12-812297-6

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Abstract

Marilins mediate interactions between macromolecular components of the extracellular matrix, e.g., collagens and proteoglycans. They are composed of von Willebrand factor type A and epidermal growth factor-like domains and the subunits oligomerize via coiled-coil domains. Matrilin-1 and -3 are abundant in hyaline cartilage, whereas matrilin-2 and -4 are widespread but less abundant. Mutations in matrilin genes have been linked to chondrodysplasias and osteoarthritis and recently characterization of matrilin-deficient mice revealed novel functions in mechanotransduction, regeneration, or inflammation. Due to their intrinsic adhesiveness and partially also low abundance, the study of matrilins is cumbersome. In this chapter, we describe methods for purification of matrilins from tissue, analysis of matrilins in tissue extracts, recombinant expression, and generation of matrilin-specific antibodies.

Item Type:	Book Section, Proceedings Item or annotation in a legal commentary
Creators:	Creators Email ORCID ORCID Put Code Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener, Raimund UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-202656
DOI:	10.1016/bs.mcb.2017.08.018
Title of Book:	Methods in Cell Biology
Series Name:	Methods Cell Biol.
Volume:	143
Page Range:	S. 429 - 447
Date:	2018
Publisher:	ELSEVIER ACADEMIC PRESS INC
Place of Publication:	SAN DIEGO
ISSN:	0091-679X
ISBN:	978-0-12-812297-6
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MULTIPLE EPIPHYSEAL DYSPLASIA; VON-WILLEBRAND-FACTOR; 148-KILODALTON CARTILAGE PROTEIN; EXTRACELLULAR-MATRIX; MICE LACKING; COILED-COIL; SKELETAL DEVELOPMENT; HAND OSTEOARTHRITIS; MOLECULAR-STRUCTURE; TISSUE DISTRIBUTION Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20265