Universität zu Köln

Uribe, Pamela, Rojas, Christian, Merino, Juan, Zambrano, Fabiola, Villegas, Juana V., Treulen, Favian, Boguen, Rodrigo ORCID: 0000-0002-0325-743X, Isachenko, Vladimir, Isachenko, Evgenia and Sanchez, Raul (2017). Effect of incubation temperature after devitrification on quality parameters in human sperm cells. Cryobiology, 79. S. 78 - 82. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2392

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Abstract

Sperm cryopreservation is common in assisted reproduction laboratories, providing a therapeutic option for several clinical conditions. This process has been optimized; however, the effect of post-thaw incubation temperature has been poorly studied. This work analyzed the effect of incubation temperature after devitrification on human sperm quality. Spermatozoa from normozoospermic donors were cryopreserved by vitrification. After devitrification, the spermatozoa were separated into two aliquots: (i) incubated at room temperature (RT, 22-25 degrees C) and (ii) incubated at 37 degrees C. Reactive oxygen species (ROS), viability, mitochondrial membrane potential (AWM), phosphatidylserine externalization and motility were analyzed immediately after devitrification (control) and after 2, 4 and 6 h. Spermatozoa incubated at RT showed a conserved viability and Delta Psi M compared to the control, while the incubation at 37 degrees C promoted a decrease in these parameters. The ROS levels were increased at both incubation conditions. The progressive motility was decreased in all experimental groups and the decrease was more pronounced under incubation at RT. No increase in phosphatidylserine externalization was observed. In conclusion, prior to use in assisted reproduction procedures, devitrified spermatozoa at RT conserve a better viability and Delta Psi M than at 37 degrees C.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Uribe, Pamela UNSPECIFIED UNSPECIFIED UNSPECIFIED Rojas, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Merino, Juan UNSPECIFIED UNSPECIFIED UNSPECIFIED Zambrano, Fabiola UNSPECIFIED UNSPECIFIED UNSPECIFIED Villegas, Juana V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Treulen, Favian UNSPECIFIED UNSPECIFIED UNSPECIFIED Boguen, Rodrigo UNSPECIFIED orcid.org/0000-0002-0325-743X UNSPECIFIED Isachenko, Vladimir UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Sanchez, Raul UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-210069
DOI:	10.1016/j.cryobiol.2017.10.005
Journal or Publication Title:	Cryobiology
Volume:	79
Page Range:	S. 78 - 82
Date:	2017
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2392
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FERTILITY PRESERVATION; HUMAN SPERMATOZOA; CRYOPRESERVATION; VITRIFICATION; INFERTILITY; MOTILITY; CRYOPROTECTANTS; APOPTOSIS; TEMPOL Multiple languages Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21006