Rios-Barrera, L. D., Sigurbjoernsdottir, S., Baer, M. and Leptin, M. (2017). Dual function for Tango1 in secretion of bulky cargo and in ER-Golgi morphology. Proc. Natl. Acad. Sci. U. S. A., 114 (48). S. E10389 - 10. WASHINGTON: NATL ACAD SCIENCES. ISSN 0027-8424

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Abstract

Tango1 enables ER-to-Golgi trafficking of large proteins. We show here that loss of Tango1, in addition to disrupting protein secretion and ER/Golgi morphology, causes ER stress and defects in cell shape. We find that the previously observed dependence of smaller cargos on Tango1 is a secondary effect. If large cargos like Dumpy, which we identify as a Tango1 cargo, are removed from the cell, nonbulky proteins reenter the secretory pathway. Removal of blocking cargo also restores cell morphology and attenuates the ER-stress response. Thus, failures in the secretion of nonbulky proteins, ER stress, and defective cell morphology are secondary consequences of bulky cargo retention. By contrast, ER/Golgi defects in Tango1-depleted cells persist in the absence of bulky cargo, showing that they are due to a secretion-independent function of Tango1. Therefore, maintenance of ER/Golgi architecture and bulky cargo transport are the primary functions for Tango1.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Rios-Barrera, L. D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sigurbjoernsdottir, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baer, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leptin, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-210891
DOI: 10.1073/pnas.1711408114
Journal or Publication Title: Proc. Natl. Acad. Sci. U. S. A.
Volume: 114
Number: 48
Page Range: S. E10389 - 10
Date: 2017
Publisher: NATL ACAD SCIENCES
Place of Publication: WASHINGTON
ISSN: 0027-8424
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EXTRACELLULAR-MATRIX; COLLAGEN SECRETION; EXIT SITES; DROSOPHILA; CTAGE5; MORPHOGENESIS; MECHANISMS; TRANSPORT; DEFECTS; SPARCMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/21089

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