Universität zu Köln

Linares-Otoya, Luis ORCID: 0000-0001-7066-5452, Linares-Otoya, Virginia, Armas-Mantilla, Lizbeth, Blanco-Olano, Cyntia, Cruesemann, Max, Ganoza-Yupanqui, Mayar L., Campos-Florian, Julio, Koenig, Gabriele M. and Schaeberle, Till F. (2017). Identification and heterologous expression of the kocurin biosynthetic gene cluster. Microbiology-(UK), 163 (10). S. 1409 - 1415. LONDON: MICROBIOLOGY SOC. ISSN 1465-2080

Full text not available from this repository.

Abstract

The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with activity against methicillin-resistant Staphylococcus aureus (MRSA) strains was revealed by comparative genome analysis of this new isolate and other reported thiopeptide producer strains. The functionality of the predicted gene locus was experimentally proven by heterologous expression in Streptomyces coelicolor M1146. Expression of the gene cluster under the control of a constitutive promoter enabled the transgenic strain to produce kocurin in selected media. The kocurin biosynthetic gene cluster comprises nine open reading frames and spans around 12 kbp of the genome.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Linares-Otoya, Luis UNSPECIFIED orcid.org/0000-0001-7066-5452 UNSPECIFIED Linares-Otoya, Virginia UNSPECIFIED UNSPECIFIED UNSPECIFIED Armas-Mantilla, Lizbeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Blanco-Olano, Cyntia UNSPECIFIED UNSPECIFIED UNSPECIFIED Cruesemann, Max UNSPECIFIED UNSPECIFIED UNSPECIFIED Ganoza-Yupanqui, Mayar L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Campos-Florian, Julio UNSPECIFIED UNSPECIFIED UNSPECIFIED Koenig, Gabriele M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaeberle, Till F. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-215778
DOI:	10.1099/mic.0.000538
Journal or Publication Title:	Microbiology-(UK)
Volume:	163
Number:	10
Page Range:	S. 1409 - 1415
Date:	2017
Publisher:	MICROBIOLOGY SOC
Place of Publication:	LONDON
ISSN:	1465-2080
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language THIOPEPTIDE ANTIBIOTICS; THIAZOLYL PEPTIDE; ANTIBACTERIAL; MATURATION; BACTERIA; PM181104; GE2270 Multiple languages Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21577