Universität zu Köln

Berndt, Nicole, Seib, Ekaterina, Kim, Soya, Troost, Tobias, Lyga, Marvin, Langenbach, Jessica, Haensch, Sebastian, Kalodimou, Konstantina, Delidakis, Christos ORCID: 0000-0003-3912-701X and Klein, Thomas (2017). Ubiquitylation-independent activation of Notch signalling by Delta. eLife, 6. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

Ubiquitylation (ubi) by the E3-ligases Mindbombl (Mib1) and Neuralized (Neur) is required for activation of the DSL ligands Delta (DI) and Serrate (Ser) to activate Notch signalling. These ligases transfer ubiquitin to lysines of the ligands' intracellular domains (ICDs), which sends them into an Epsin-dependent endocytic pathway. Here, we have tested the requirement of ubi of DI for signalling. We found that DI requires ubi for its full function, but can also signal in two ubi-independent modes, one dependent and one independent of Neur. We identified two neural lateral specification processes where DI signals in an ubi-independent manner. Neur, which is needed for these processes, was shown to be able to activate DI in an ubi-independent manner. Our analysis suggests that one important role of DSL protein ubi by Mib1 is their release from cis inhibitory interactions with Notch, enabling them to trans-activate Notch on adjacent cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Berndt, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Seib, Ekaterina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kim, Soya UNSPECIFIED UNSPECIFIED UNSPECIFIED Troost, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Lyga, Marvin UNSPECIFIED UNSPECIFIED UNSPECIFIED Langenbach, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Haensch, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kalodimou, Konstantina UNSPECIFIED UNSPECIFIED UNSPECIFIED Delidakis, Christos UNSPECIFIED orcid.org/0000-0003-3912-701X UNSPECIFIED Klein, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-217292
DOI:	10.7554/eLife.27346
Journal or Publication Title:	eLife
Volume:	6
Date:	2017
Publisher:	ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2050-084X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language WING IMAGINAL DISC; DROSOPHILA DEVELOPMENT; ENDOCYTIC PATHWAY; UBIQUITIN LIGASE; MIND BOMB; INTRACELLULAR MOTIFS; COMPARTMENT BOUNDARY; NEURALIZED FUNCTIONS; TRANSCRIPTION FACTOR; CIS-INHIBITION Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/21729