Universität zu Köln

Ladavac, Ranko, Bedenic, Branka, Vranic-Ladavac, Mirna, Barisic, Nada, Karcic, Natalie, Pompe, Karoline, Ferencic, Antun ORCID: 0000-0002-2166-7264, Stojanovic, Aleksandar, Seifert, Harald, Katic, Stjepan and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2017). Emergence of different Acinetobacter baumannii clones in a Croatian hospital and correlation with antibiotic susceptibility. J. Glob. Antimicrob. Resist., 10. S. 213 - 219. OXFORD: ELSEVIER SCI LTD. ISSN 2213-7173

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Abstract

Objectives: During routine diagnostic laboratory work, the clinical microbiologist observed an increase of Acinetobacter baumannii isolates with three different carbapenem susceptibility patterns: susceptible, intermediate and resistant. Isolates belonging to the same carbapenem susceptibility phenotype exhibited identical susceptibility/resistance patterns to non-beta-lactam antibiotics. This prompted us to analyse the mechanisms of carbapenem-resistance and the molecular epidemiology of the isolates. A total of 59 A. baumannii isolates were analysed and grouped according to their susceptibility to imipenem: group 1 were susceptible (N = 24), group 2 were intermediate (N = 8) and group 3 were resistant (N = 27) to imipenem. Material and methods: PCR and sequencing was used to detect resistance genes. Genotyping of the isolates was performed by PFGE and MLST. Results: Out of 27 resistant isolates, 20 harboured bla(OXA)-40-like and 7 bla(OXA)-23-like genes. ISAba1 was found upstream of bla(OXA)-51 and bla(OXA)-23 genes. PFGE genotyping demonstrated the existence of three major A. baumannii clones in GH Pula and determination of sequence groups showed that the isolates belonged to international clones commonly associated with multidrug-resistance. MLST (performed on six isolates) showed diverse population structure of isolates belonging to the same cluster, including ST 195, ST 231, ST 775 and ST 1095. Conclusions: A previous study conducted in 2009-2010 showed that reduced susceptibility to carbapenems in GH Pula was only associated with upregulation of the intrinsic OXA-51 beta-lactamase. In this study a shift to isolates with acquired oxacillinases, belonging to two major clones was reported. (C) 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ladavac, Ranko UNSPECIFIED UNSPECIFIED UNSPECIFIED Bedenic, Branka UNSPECIFIED UNSPECIFIED UNSPECIFIED Vranic-Ladavac, Mirna UNSPECIFIED UNSPECIFIED UNSPECIFIED Barisic, Nada UNSPECIFIED UNSPECIFIED UNSPECIFIED Karcic, Natalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Pompe, Karoline UNSPECIFIED UNSPECIFIED UNSPECIFIED Ferencic, Antun UNSPECIFIED orcid.org/0000-0002-2166-7264 UNSPECIFIED Stojanovic, Aleksandar UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Katic, Stjepan UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-220867
DOI:	10.1016/j.jgar.2017.07.001
Journal or Publication Title:	J. Glob. Antimicrob. Resist.
Volume:	10
Page Range:	S. 213 - 219
Date:	2017
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	2213-7173
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPECTRUM BETA-LACTAMASES; CARBAPENEM RESISTANCE; MULTIPLEX PCR; OXA-23 CARBAPENEMASE; OUTBREAK; SPREAD; STRAINS; GENES; ETEST Multiple languages Infectious Diseases; Pharmacology & Pharmacy Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22086