Universität zu Köln

Sabashnikov, Anton ORCID: 0000-0002-6289-1035, Neef, Klaus, Chesnokova, Vera, Wegener, Leonie, Godthardt, Kathrin, Scherner, Maximilian, Kuhn, Elmar W., Deppe, Antje-Christin, Lauer, Meike, Eghbalzadeh, Kaveh, Zeriouh, Mohamed, Rahmanian, Parwis B. ORCID: 0000-0002-3978-9251, Wippermann, Jens, Kuhn-Regnier, Ferdinand, Madershahian, Navid, Wahlers, Thorsten, Weymann, Alexander and Choi, Yeong-Hoon (2017). Enumeration of circulating endothelial cell frequency as a diagnostic marker in aortic valve surgery - a flow cytometric approach. J. Cardiothorac. Surg., 12. LONDON: BIOMED CENTRAL LTD. ISSN 1749-8090

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Abstract

Background: The frequency of circulating endothelial cells (CEC) in patients' peripheral blood can be assessed as a direct marker of endothelial damage. However, conventional enumeration methods are extremely challenging. We developed a novel, automated approach to determine CEC frequencies and tested this method on two groups of patients undergoing conventional (CAVR) versus trans-catheter aortic valve implantation (TAVI). Methods: CEC frequencies were assessed by a flow cytometric approach, including automated pre-enrichment of CD34 positive blood cell subpopulation and isotype controls. The efficacy and reproducibility of the CEC enumeration method was validated by spiking blood samples of healthy control donors with defined numbers of endothelial cells. Results: CEC frequencies were significantly higher in the TAVI group before (9.8 +/- 4.1 vs. 5.5 +/- 2.2, p = 0.019) and 1 h after surgery (13.4 +/- 5.1 vs. 8.2 +/- 4.1, p = 0.030) corresponding to higher Euroscore, STS score in higher risk patients from the TAVI group. Five days after surgery, CEC frequencies became significantly higher in the more invasive CAVR group (39.0 +/- 13.0 vs. 14.3 +/- 4.4, p < 0.001) compared to minimally invasive TAVI approach. Conclusions: The new flow cytometric approach might be a robust and reliable method for CEC enumeration. Initial results show that CEC frequency is a valid clinical marker for the assessment of pre-operative risk, invasiveness of surgical procedure and clinical outcome. Further studies are necessary to validate the practical clinical usefulness and the potential superiority compared to conventional markers.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sabashnikov, Anton UNSPECIFIED orcid.org/0000-0002-6289-1035 UNSPECIFIED Neef, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Chesnokova, Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED Wegener, Leonie UNSPECIFIED UNSPECIFIED UNSPECIFIED Godthardt, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherner, Maximilian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhn, Elmar W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Deppe, Antje-Christin UNSPECIFIED UNSPECIFIED UNSPECIFIED Lauer, Meike UNSPECIFIED UNSPECIFIED UNSPECIFIED Eghbalzadeh, Kaveh UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeriouh, Mohamed UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahmanian, Parwis B. UNSPECIFIED orcid.org/0000-0002-3978-9251 UNSPECIFIED Wippermann, Jens UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhn-Regnier, Ferdinand UNSPECIFIED UNSPECIFIED UNSPECIFIED Madershahian, Navid UNSPECIFIED UNSPECIFIED UNSPECIFIED Wahlers, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Weymann, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-221855
DOI:	10.1186/s13019-017-0631-3
Journal or Publication Title:	J. Cardiothorac. Surg.
Volume:	12
Date:	2017
Publisher:	BIOMED CENTRAL LTD
Place of Publication:	LONDON
ISSN:	1749-8090
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VON-WILLEBRAND-FACTOR; ACUTE CORONARY SYNDROMES; PERIPHERAL-BLOOD; PROGENITOR CELLS; CARDIOPULMONARY BYPASS; VASCULAR DISORDERS; DIABETES-MELLITUS; INJURY; DAMAGE; CANCER Multiple languages Cardiac & Cardiovascular Systems; Surgery Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22185