Gedik, Nilguen, Krueger, Marcus, Thielmann, Matthias, Kottenberg, Eva, Skyschally, Andreas, Frey, Ulrich H., Cario, Elke, Peters, Juergen, Jakob, Heinz, Heusch, Gerd and Kleinbongard, Petra (2017). Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning. Sci Rep, 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2045-2322

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Abstract

Remote ischemic preconditioning (RIPC) by repeated brief cycles of limb ischemia/reperfusion reduces myocardial ischemia/reperfusion injury. In left ventricular (LV) biopsies from patients undergoing coronary artery bypass grafting (CABG), only the activation of signal transducer and activator of transcription 5 was associated with RIPC's cardioprotection. We have now used an unbiased, non-hypothesis-driven proteomics and phosphoproteomics approach to analyze LV biopsies from patients undergoing CABG and from pigs undergoing coronary occlusion/reperfusion without (sham) and with RIPC. False discovery rate-based statistics identified a higher prostaglandin reductase 2 expression at early reperfusion with RIPC than with sham in patients. In pigs, the phosphorylation of 116 proteins was different between baseline and early reperfusion with RIPC and/or with sham. The identified proteins were not identical for patients and pigs, but in-silico pathway analysis of proteins with >= 2-fold higher expression/phosphorylation at early reperfusion with RIPC in comparison to sham revealed a relation to mitochondria and cytoskeleton in both species. Apart from limitations of the proteomics analysis per se, the small cohorts, the sampling/sample processing and the number of uncharacterized/unverifiable porcine proteins may have contributed to this largely unsatisfactory result.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gedik, NilguenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thielmann, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kottenberg, EvaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Skyschally, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Frey, Ulrich H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cario, ElkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peters, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jakob, HeinzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heusch, GerdUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kleinbongard, PetraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-221878
DOI: 10.1038/s41598-017-07883-5
Journal or Publication Title: Sci Rep
Volume: 7
Date: 2017
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2045-2322
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ELEVATION MYOCARDIAL-INFARCTION; PRESERVES MITOCHONDRIAL-FUNCTION; CARDIOLOGY WORKING GROUP; INJURY SALVAGE KINASE; BYPASS GRAFT-SURGERY; K-ATP CHANNELS; REPERFUSION INJURY; ISCHEMIA/REPERFUSION INJURY; SIGNAL-TRANSDUCTION; CALPAIN ACTIVATIONMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22187

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