Friede, Alexandra, Zhang, Bipei, Herberth, Stefanie, Pesch, Martina, Schrader, Andrea ORCID: 0000-0002-3879-7057 and Huelskamp, Martin (2017). The Second Intron Is Essential for the Transcriptional Control of the Arabidopsis thaliana GLABRA3 Gene in Leaves. Front. Plant Sci., 8. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

The GLABRA3 gene is a major regulator of trichome patterning in Arabidopsis thaliana. The regulatory regions important for the trichome-specific expression of GL3 have not been characterized yet. In this study, we used a combination of marker and rescue constructs to determine the relevant promoter regions. We demonstrate that a 1 kb 5' region combined with the second intron is sufficient to rescue the trichome mutant phenotype of gl3 egl3 mutants. Swap experiments of the second intron suggest that it is not sufficient to generally enhance the expression level of GL3. This implies that the second intron contains regulatory regions for the temporal and spatial regulation of GL3. The corresponding GUS-marker constructs revealed trichome-specific expression in young trichomes.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Friede, AlexandraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, BipeiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Herberth, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pesch, MartinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schrader, AndreaUNSPECIFIEDorcid.org/0000-0002-3879-7057UNSPECIFIED
Huelskamp, MartinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-221886
DOI: 10.3389/fpls.2017.01382
Journal or Publication Title: Front. Plant Sci.
Volume: 8
Date: 2017
Publisher: FRONTIERS MEDIA SA
Place of Publication: LAUSANNE
ISSN: 1664-462X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPIDERMAL-CELL DIFFERENTIATION; TRICHOME DEVELOPMENT; REGULATORY EVENTS; ROOT; GL3; PROTEIN; EXPRESSION; INITIATION; ENHANCER; ENCODESMultiple languages
Plant SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22188

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