Universität zu Köln

Paeger, Lars ORCID: 0000-0001-8716-3483, Pippow, Andreas, Hess, Simon, Paehler, Moritz, Klein, Andreas C., Husch, Andreas, Pouzat, Christophe ORCID: 0000-0002-2844-8099, Bruening, Jens C. and Kloppenburg, Peter (2017). Energy imbalance alters Ca2+ handling and excitability of POMC neurons. eLife, 6. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD. ISSN 2050-084X

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Abstract

Satiety-signaling, pro-opiomelanocortin (POMC)-expressing neurons in the arcuate nucleus of the hypothalamus play a pivotal role in the regulation of energy homeostasis. Recent studies reported altered mitochondrial dynamics and decreased mitochondria-endoplasmic reticulum contacts in POMC neurons during diet-induced obesity. Since mitochondria play a crucial role in Ca2+ signaling, we investigated whether obesity alters Ca2+ handling of these neurons in mice. In diet-induced obesity, cellular Ca2+ handling properties including mitochondrial Ca2+ uptake capacity are impaired, and an increased resting level of free intracellular Ca2+ is accompanied by a marked decrease in neuronal excitability. Experimentally increasing or decreasing intracellular Ca2+ concentrations reproduced electrophysiological properties observed in diet-induced obesity. Taken together, we provide the first direct evidence for a diet-dependent deterioration of Ca2+ homeostasis in POMC neurons during obesity development resulting in impaired function of these critical energy homeostasis-regulating neurons.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Paeger, Lars UNSPECIFIED orcid.org/0000-0001-8716-3483 UNSPECIFIED Pippow, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Hess, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Paehler, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Andreas C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Husch, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Pouzat, Christophe UNSPECIFIED orcid.org/0000-0002-2844-8099 UNSPECIFIED Bruening, Jens C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloppenburg, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-223409
DOI:	10.7554/eLife.25641
Journal or Publication Title:	eLife
Volume:	6
Date:	2017
Publisher:	ELIFE SCIENCES PUBLICATIONS LTD
Place of Publication:	CAMBRIDGE
ISSN:	2050-084X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIVATED POTASSIUM CHANNELS; SPIKE-FREQUENCY ADAPTATION; ENDOPLASMIC-RETICULUM STRESS; LEPTIN RESISTANCE; SMALL-CONDUCTANCE; K+ CHANNELS; INTERMEDIATE-CONDUCTANCE; HYPOTHALAMIC NEURONS; GLUCOSE-HOMEOSTASIS; OBESITY EPIDEMIC Multiple languages Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22340