Winkler, Martin ORCID: 0000-0002-6196-6381, Niemeyer, Michael ORCID: 0000-0001-7501-8879, Hellmuth, Antje, Janitza, Philipp, Christ, Gideon, Samodelov, Sophia L., Wilde, Verona, Majovsky, Petra, Trujillo, Marco ORCID: 0000-0002-5470-7277, Zurbriggen, Matias D., Hoehenwarter, Wolfgang ORCID: 0000-0002-7669-7524, Quint, Marcel ORCID: 0000-0003-2935-4083 and Villalobos, Luz Irina A. Calderon (2017). Variation in auxin sensing guides AUX/IAA transcriptional repressor ubiquitylation and destruction. Nat. Commun., 8. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Auxin is a small molecule morphogen that bridges SCFTIR1/AFB-AUX/IAA co-receptor interactions leading to ubiquitylation and proteasome-dependent degradation of AUX/IAA transcriptional repressors. Here, we systematically dissect auxin sensing by SCFTIR1-IAA6 and SCFTIR1-IAA19 co-receptor complexes, and assess IAA6/IAA19 ubiquitylation in vitro and IAA6/IAA19 degradation in vivo. We show that TIR1-IAA19 and TIR1-IAA6 have distinct auxin affinities that correlate with ubiquitylation and turnover dynamics of the AUX/IAA. We establish a system to track AUX/IAA ubiquitylation in IAA6 and IAA19 in vitro and show that it occurs in flexible hotspots in degron-flanking regions adorned with specific Lys residues. We propose that this signature is exploited during auxin-mediated SCFTIR1-AUX/IAA interactions. We present evidence for an evolving AUX/IAA repertoire, typified by the IAA6/IAA19 ohnologues, that discriminates the range of auxin concentrations found in plants. We postulate that the intrinsic flexibility of AUX/IAAs might bias their ubiquitylation and destruction kinetics enabling specific auxin responses.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Winkler, MartinUNSPECIFIEDorcid.org/0000-0002-6196-6381UNSPECIFIED
Niemeyer, MichaelUNSPECIFIEDorcid.org/0000-0001-7501-8879UNSPECIFIED
Hellmuth, AntjeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Janitza, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Christ, GideonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Samodelov, Sophia L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wilde, VeronaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Majovsky, PetraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Trujillo, MarcoUNSPECIFIEDorcid.org/0000-0002-5470-7277UNSPECIFIED
Zurbriggen, Matias D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoehenwarter, WolfgangUNSPECIFIEDorcid.org/0000-0002-7669-7524UNSPECIFIED
Quint, MarcelUNSPECIFIEDorcid.org/0000-0003-2935-4083UNSPECIFIED
Villalobos, Luz Irina A. CalderonUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-227926
DOI: 10.1038/ncomms15706
Journal or Publication Title: Nat. Commun.
Volume: 8
Date: 2017
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
BOX PROTEIN TIR1; STRUCTURAL BASIS; REGULATED PROTEIN; MOLECULAR-BASIS; GENE FAMILY; DEGRADATION; UBIQUITINATION; SPECIFICITY; E2; EXPRESSIONMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/22792

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