Universität zu Köln

Sabour, Davood, Srinivasan, Sureshkumar Perumal, Rohani, Susan, Wagh, Vilas, Gaspar, John Antonydas, Panek, Darius, Ardestani, Mostafa Abootorabi, Doss, Michael Xavier, Riet, Nicole, Abken, Hinrich, Hescheler, Juergen, Papadopoulos, Symeon and Sachinidis, Agapios (2017). STRIP2 Is Indispensable for the Onset of Embryonic Stem Cell Differentiation. Mol.Ther.-Methods Clin. Dev., 5. S. 116 - 130. CAMBRIDGE: CELL PRESS. ISSN 2329-0501

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Abstract

The role of striatin interacting protein 2 (Strip2) in differentiation of embryonic stem cells (ESCs) is still under debate. Strip2-silenced murine (KD) ESCs were differentiated for 4, 8, 12, and 16 days. We show that Strip2 is distributed in the perinucleus or nuclei of wild-type (WT) undifferentiated ESCs, but is localized in high-density nuclear bodies in differentiated cells. CellNet analysis of microarray gene expression data for the KD and scrambled control (SCR) embryoid bodies (EBs), as well as immunostainings of key pluripotent factors, demonstrated that differentiation of KD ESCs is repressed. This occurs even in 16-day-old EBs, which possessed a high tumorigenic potential. Correlated with very high expression levels of epigenetic regulator genes, Hat1 and Dnmt3, enzymatic activities of the histone acetyltransferase type B (Hat1) and DNA (cytosine-5)-methyltransferase 3 beta (Dnmt3b) were higher in differentiated 16-day-old KD EBs than in SCR or WT EBs. The expression levels of let-7, 290, and 302 microRNA families were opposed in KD ESCs, while KD EBs had levels comparable to WT and SCR ESCs during differentiation. Strip2 is critical for the regular differentiation of ESCs. Moreover, Strip2 deficient ESCs showed a dysregulation of epigenetic regulators and microRNAs regulating pluripotency.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sabour, Davood UNSPECIFIED UNSPECIFIED UNSPECIFIED Srinivasan, Sureshkumar Perumal UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohani, Susan UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagh, Vilas UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaspar, John Antonydas UNSPECIFIED UNSPECIFIED UNSPECIFIED Panek, Darius UNSPECIFIED UNSPECIFIED UNSPECIFIED Ardestani, Mostafa Abootorabi UNSPECIFIED UNSPECIFIED UNSPECIFIED Doss, Michael Xavier UNSPECIFIED UNSPECIFIED UNSPECIFIED Riet, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Abken, Hinrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Papadopoulos, Symeon UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-228377
DOI:	10.1016/j.omtm.2017.04.001
Journal or Publication Title:	Mol.Ther.-Methods Clin. Dev.
Volume:	5
Page Range:	S. 116 - 130
Date:	2017
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2329-0501
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EPIGENETIC REGULATION; EXPRESSION; PLURIPOTENCY; ORGANIZATION; METHYLATION; BIOGENESIS; REGULATORS; COMPLEXES; CHROMATIN; GENE Multiple languages Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/22837