Universität zu Köln

Auler, Markus, Pitzler, Lena, Poeschi, Ernst, Zhou, Zhigang and Brachvogel, Bent (2017). Mimicking Angiogenesis in vitro: Three-dimensional Co-culture of Vascular Endothelial Cells and Perivascular Cells in Collagen Type I Gels. Bio-protocol, 7 (8). SUNNYVALE: BIO-PROTOCOL. ISSN 2331-8325

Full text not available from this repository.

Abstract

Angiogenesis defines the process of formation of new vascular structures form existing blood vessels, involved during development, repair processes like wound healing but also linked to pathological changes. During angiogenic processes, endothelial cells build a vascular network and recruit perivascular cells to form mature, stable vessels. Endothelial cells and perivascular cells secret and assemble a vascular basement membrane and interact via close cell-cell contacts. To mimic these processes in vitro we have developed a versatile three-dimensional culture system where perivascular cells (PVC) are co-cultured with human umbilical cord vascular endothelial cells (HUVEC) in a collagen type I gel. This co-culture system can be used to determine biochemical and cellular processes during neoangiogenic events with a wide range of analyses options.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Auler, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Pitzler, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Poeschi, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhou, Zhigang UNSPECIFIED UNSPECIFIED UNSPECIFIED Brachvogel, Bent UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-233807
DOI:	10.21769/BioProtoc.2247
Journal or Publication Title:	Bio-protocol
Volume:	7
Number:	8
Date:	2017
Publisher:	BIO-PROTOCOL
Place of Publication:	SUNNYVALE
ISSN:	2331-8325
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23380