Universität zu Köln

Rahn, Elena, Thier, Katharina, Petermann, Philipp, Ruebsam, Matthias, Staeheli, Peter, Iden, Sandra ORCID: 0000-0003-2333-9827, Niessen, Carien M. and Knebel-Moersdorf, Dagmar (2017). Epithelial Barriers in Murine Skin during Herpes Simplex Virus 1 Infection: The Role of Tight Junction Formation. J. Invest. Dermatol., 137 (4). S. 884 - 894. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

Herpes simplex virus 1 has to overcome skin or mucosa barriers to infect its human host. The impact of the various barrier functions on successful viral invasion is not known. On ex vivo infection of murine skin, we observed efficient invasion only via the basal epidermal layer when the dermis was removed. Here, we investigated how wounding and intercellular junction formation control successful viral entry. After wounding of skin samples or removal of the stratum corneum, infected cells were rarely detected. On the basis of infection studies in epidermis from IFN-stimulated mice, we assume that mechanical wounding does not lead to an antiviral state that impedes infection. When we infected human skin equivalents, we observed entry only into unstratified keratinocytes or after wounding of fully stratified cultures. Reduced infection of keratinocytes after calcium-induced stratification confirmed the impact of junction formation. To assess the effect of functional tight junctions, stratified cultures of polarity regulator partitioning-defective-3-or E-cadherin-deficient keratinocytes were infected. As the number of infected cells strongly increased with enhanced paracellular permeability, we conclude that the formation of functional tight junctions interferes with viral entry indicating that next to the stratum corneum tight junctions are a major physical barrier for herpes simplex virus 1 invasion into tissue.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rahn, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Thier, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Petermann, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruebsam, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Staeheli, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Iden, Sandra UNSPECIFIED orcid.org/0000-0003-2333-9827 UNSPECIFIED Niessen, Carien M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Knebel-Moersdorf, Dagmar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-236173
DOI:	10.1016/j.jid.2016.11.027
Journal or Publication Title:	J. Invest. Dermatol.
Volume:	137
Number:	4
Page Range:	S. 884 - 894
Date:	2017
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1523-1747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language KERATINOCYTE CELL-LINE; ADHERENS JUNCTIONS; EPIDERMAL BARRIER; ENTRY; TYPE-1; NECTIN-1; RECEPTOR; CADHERIN; PROTEIN Multiple languages Dermatology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/23617