Andon, Fernando T., Mukherjee, Sourav P., Gessner, Isabel, Wortmann, Laura, Xiao, Lisong, Hultenby, Kjell, Shvedova, Anna A., Mathur, Sanjay and Fadeel, Bengt (2017). Hollow carbon spheres trigger inflammasome-dependent IL-1 beta secretion in macrophages. Carbon, 113. S. 243 - 252. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-3891

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Abstract

It is disputed whether inflammasome activation leading to secretion of pro-inflammatory interleukin (IL)-1 beta in macrophages transpires independently of cell death or whether the two processes are linked. Here, we synthesized hollow carbon spheres (HCS) and investigated their effects on primary human monocyte-derived macrophages (HMDM); short (500 nm) non-functionalized single-walled carbon nanotubes (SWCNT) were included for comparison. HCS (250 nm) were readily taken up by HMDM and induced ROS production, but did not trigger a loss of cell viability. However, a dose- and time-dependent release of IL-1 beta was detected in lipopolysaccharide (LPS)-primed macrophages upon exposure to HCS, while SWCNT-induced secretion of IL-1 beta was less pronounced. HCS-triggered IL-1 beta secretion was cathepsin B- and caspase-1-dependent, and was accompanied by a reduction in intracellular K+. Furthermore, cytokine secretion was reduced following treatment with the antioxidant, N-acetylcysteine, and cytochalasin D, an inhibitor of actin polymerization. HCS also triggered IL-1 beta release in LPS-primed THP.1 cells, but not'in THP.1 cells with silencing of ASC, NLRP3, or caspase-1 expression, providing evidence that IL-1 beta was elicited through NLRP3 inflammasome activation. These studies shed light on the effects of HCS on primary macrophages, and show that spherical carbon-based nanoparticles are potent inflammasome activators. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Andon, Fernando T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mukherjee, Sourav P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gessner, IsabelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wortmann, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xiao, LisongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hultenby, KjellUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Shvedova, Anna A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathur, SanjayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fadeel, BengtUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-239409
DOI: 10.1016/j.carbon.2016.11.049
Journal or Publication Title: Carbon
Volume: 113
Page Range: S. 243 - 252
Date: 2017
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication: OXFORD
ISSN: 1873-3891
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
INDUCED TOXICITY FOCUS; NLRP3 INFLAMMASOME; BIOLOGICAL INTERACTIONS; PULMONARY INFLAMMATION; OXIDE NANOPARTICLES; NALP3 INFLAMMASOME; STEM-CELLS; ACTIVATION; NANOTUBES; NANOMATERIALSMultiple languages
Chemistry, Physical; Materials Science, MultidisciplinaryMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/23940

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