Universität zu Köln

van Beek, Adriaan A., Sovran, Bruno, Hugenholtz, Floor, Meijer, Ben, Hoogerland, Joanne A., Mihailova, Violeta, van der Ploeg, Corine, Belzer, Clara, Boekschoten, Mark V., Hoeijmakers, Jan H. J., Vermeij, Wilbert P., de Vos, Paul, Wells, Jerry M., Leenen, Pieter J. M., Nicoletti, Claudio, Hendriks, Rudi W. and Savelkoul, Huub F. J. (2016). Supplementation with Lactobacillus plantarum WCFS1 Prevents Decline of Mucus Barrier in Colon of Acclerated Aging Ercc1(-/Delta 7) Mice. Front. Immunol., 7. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-3224

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Abstract

Although it is clear that probiotics improve intestinal barrier function, little is known about the effects of probiotics on the aging intestine. We investigated effects of 10-week bacterial supplementation of Lactobacillus plantarum WCFS1, Lactobacillus casei BL23, or Bifidobacterium breve DSM20213 on gut barrier and immunity in 16-week-old accelerated aging Ercc1(-/Delta 7) mice, which have a median lifespan of similar to 20 weeks, and their wild-type littermates. The colonic barrier in Ercc1(-/Delta 7) mice was characterized by a thin (< 10 mu m) mucus layer. L. plantarum prevented this decline in mucus integrity in Ercc1(-/Delta 7) mice, whereas B. breve exacerbated it. Bacterial supplementations affected the expression of immune-related genes, including Toll-like receptor 4. Regulatory T cell frequencies were increased in the mesenteric lymph nodes of L. plantarum- and L. casei-treated Ercc1(-/Delta 7) mice. L. plantarum- and L. casei-treated Ercc1(-/Delta 7) mice showed increased specific antibody production in a T cell-dependent immune response in vivo. By contrast, the effects of bacterial supplementation on wild-type control mice were negligible. Thus, supplementation with L. plantarum - but not with L. casei and B. breve - prevented the decline in the mucus barrier in Ercc1(-/Delta 7) mice. Our data indicate that age is an important factor influencing beneficial or detrimental effects of candidate probiotics. These findings also highlight the need for caution in translating beneficial effects of probiotics observed in young animals or humans to the elderly.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code van Beek, Adriaan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sovran, Bruno UNSPECIFIED UNSPECIFIED UNSPECIFIED Hugenholtz, Floor UNSPECIFIED UNSPECIFIED UNSPECIFIED Meijer, Ben UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoogerland, Joanne A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mihailova, Violeta UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Ploeg, Corine UNSPECIFIED UNSPECIFIED UNSPECIFIED Belzer, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Boekschoten, Mark V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoeijmakers, Jan H. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vermeij, Wilbert P. UNSPECIFIED UNSPECIFIED UNSPECIFIED de Vos, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Wells, Jerry M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Leenen, Pieter J. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nicoletti, Claudio UNSPECIFIED UNSPECIFIED UNSPECIFIED Hendriks, Rudi W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Savelkoul, Huub F. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-258531
DOI:	10.3389/fimmu.2016.00408
Journal or Publication Title:	Front. Immunol.
Volume:	7
Date:	2016
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-3224
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO; MICROBIOTA; IDENTIFICATION; PROTEINS; MUCIN; RECOGNITION; HOMEOSTASIS; MECHANISMS; EXPRESSION; PROBIOTICS Multiple languages Immunology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/25853