Breddermann, Hannes and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2016). Correlation of Antagonistic Regulation of leuO Transcription with the Cellular Levels of BgIJ-RcsB and LeuO in Escherichia coil. Front. Cell. Infect. Microbiol., 6. LAUSANNE: FRONTIERS MEDIA SA. ISSN 2235-2988

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Abstract

LeuO is a conserved and pleiotropic transcription regulator, antagonist of the nucleoid-associated silencer protein H-NS, and important for pathogenicity and multidrug resistance in Enterobacteriaceae. Regulation of transcription of the leuo gene is complex. It is silenced by H-NS and its paralog StpA, and it is autoregulated. In addition, in Escherichia colt leuO is antagonistically regulated by the heterodimeric transcription regulator BgIJ-RcsB and by LeuO. BgIJ-RcsB activates leuO, while LeuO inhibits activation by BgIJ-RcsB. Furthermore, LeuO activates expression of bg/J, which is likewise H-NS repressed. Mutual activation of leuO and bglJ resembles a double-positive feedback network, which theoretically can result in bi-stability and heterogeneity, or be maintained in a stable OFF or ON states by an additional signal. Here we performed quantitative and single-cell expression analyses to address the antagonistic regulation and feedback control of leuO transcription by BgIJ-RcsB and LeuO using a leuO promoter m Venus reporter fusion and finely tunable bgIJ and leuO expression plasmids. The data revealed uniform regulation of leuO expression in the population that correlates with the relative cellular concentration of BgIJ and LeuO. The data are in agreement with a straightforward model of antagonistic regulation of leuO expression by the two regulators, LeuO and BgIJ-RcsB, by independent mechanisms. Further, the data suggest that at standard laboratory growth conditions feedback regulation of leuO is of minor relevance and that silencing of feu and bglJ by H-NS (and StpA) keeps these loci in the OFF state.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Breddermann, HannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schnetz, KarinUNSPECIFIEDorcid.org/0000-0003-4693-6202UNSPECIFIED
URN: urn:nbn:de:hbz:38-262257
DOI: 10.3389/fcimb.2016.00106
Journal or Publication Title: Front. Cell. Infect. Microbiol.
Volume: 6
Date: 2016
Publisher: FRONTIERS MEDIA SA
Place of Publication: LAUSANNE
ISSN: 2235-2988
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ENTERICA SEROVAR TYPHI; NS-MEDIATED REPRESSION; COLI-BGL OPERON; P-BAD PROMOTER; H-NS; GENE-EXPRESSION; GLOBAL REGULATOR; PROTEINS; SYSTEM; RNAMultiple languages
Immunology; MicrobiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/26225

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