Universität zu Köln

Hos, N. J., Wiegel, P., Fischer, J. and Plum, G. (2016). Comparative evaluation of two fully-automated real-time PCR methods for MRSA admission screening in a tertiary-care hospital. Eur. J. Clin. Microbiol. Infect. Dis., 35 (9). S. 1475 - 1479. NEW YORK: SPRINGER. ISSN 1435-4373

Full text not available from this repository.

Abstract

We evaluated two fully-automated real-time PCR systems, the novel QIAGEN artus MRSA/SA QS-RGQ and the widely used BD MAX MRSA assay, for their diagnostic performance in MRSA admission screening in a tertiary-care university hospital. Two hundred sixteen clinical swabs were analyzed for MRSA DNA using the BD MAX MRSA assay. In parallel, the same specimens were tested with the QIAGEN artus MRSA/SA QS-RGQ. Automated steps included lysis of bacteria, DNA extraction, real-time PCR and interpretation of results. MRSA culture was additionally performed as a reference method for MRSA detection. Sensitivity values were similar for both assays (80 %), while the QIAGEN artus MRSA/SA QS-RGQ reached a slightly higher specificity (95.8 % versus 90.0 %). Positive (PPVs) and negative predictive values (NPVs) were 17.4 % and 99.4 % for the BD MAX MRSA assay and 33.3 % and 99.5 % for the QIAGEN artus MRSA/SA QS-RGQ, respectively. Total turn-around time (TAT) for 24 samples was 3.5 hours for both assays. In conclusion, both assays represent reliable diagnostic tools due to their high negative predictive values, especially for the rapid identification of MRSA negative patients in a low prevalence MRSA area.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hos, N. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wiegel, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Fischer, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plum, G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-263768
DOI:	10.1007/s10096-016-2687-8
Journal or Publication Title:	Eur. J. Clin. Microbiol. Infect. Dis.
Volume:	35
Number:	9
Page Range:	S. 1475 - 1479
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-4373
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STAPHYLOCOCCUS-AUREUS; RAPID DETECTION; ASSAY; BACTEREMIA; SPECIMENS; MORTALITY; STRAINS; SAMPLES; COSTS Multiple languages Infectious Diseases; Microbiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/26376