Rehman, Syed Arif Abdul, Kristariyanto, Yosua Adi, Choi, Soo-Youn, Nkosi, Pedro Junior, Weidlich, Simone, Labib, Karim, Hofmann, Kay and Kulathu, Yogesh ORCID: 0000-0002-3274-1642 (2016). MINDY-1 Is a Member of an Evolutionarily Conserved and Structurally Distinct New Family of Deubiquitinating Enzymes. Mol. Cell, 63 (1). S. 146 - 156. CAMBRIDGE: CELL PRESS. ISSN 1097-4164

Full text not available from this repository.

Abstract

Deubiquitinating enzymes (DUBs) remove ubiquitin (Ub) from Ub-conjugated substrates to regulate the functional outcome of ubiquitylation. Here we report the discovery of a new family of DUBs, which we have named MINDY (motif interacting with Ub-containing novel DUB family). Found in all eukaryotes, MINDY-family DUBs are highly selective at cleaving K48-linked polyUb, a signal that targets proteins for degradation. We identify the catalytic activity to be encoded within a previously unannotated domain, the crystal structure of which reveals a distinct protein fold with no homology to any of the known DUBs. The crystal structure of MINDY-1 (also known as FAM63A) in complex with propargylated Ub reveals conformational changes that realign the active site for catalysis. MINDY-1 prefers cleaving long polyUb chains and works by trimming chains from the distal end. Collectively, our results reveal a new family of DUBs that may have specialized roles in regulating proteostasis.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Rehman, Syed Arif AbdulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kristariyanto, Yosua AdiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Choi, Soo-YounUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nkosi, Pedro JuniorUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weidlich, SimoneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Labib, KarimUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, KayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kulathu, YogeshUNSPECIFIEDorcid.org/0000-0002-3274-1642UNSPECIFIED
URN: urn:nbn:de:hbz:38-270093
DOI: 10.1016/j.molcel.2016.05.009
Journal or Publication Title: Mol. Cell
Volume: 63
Number: 1
Page Range: S. 146 - 156
Date: 2016
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1097-4164
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
UBIQUITIN; PROTEIN; POLYUBIQUITIN; PROTEASOME; REVEALS; RECOGNITION; SPECIFICITY; BINDING; RABEX-5Multiple languages
Biochemistry & Molecular Biology; Cell BiologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27009

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item