Koehler, Christhardt, Le, Xin, Dogan, Nasuh Utku, Pfiffer, Tatiana, Schneider, Achim, Marnitz, Simone, Bertolini, Julia and Favero, Giovanni (2016). Molecular Diagnosis for Nodal Metastasis in Endoscopically Managed Cervical Cancer: The Accuracy of the APTIMA Test to Detect High-risk Human Papillomavirus Messenger RNA in Sentinel Lymph Nodes. J. Mimim. Invasive Gynecol., 23 (5). S. 748 - 753. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1553-4669
Full text not available from this repository.Abstract
Study Objective: To evaluate the feasibility and accuracy of a commercially available test to detect E6/E7 mRNA of 14 subtypes of high-risk HPVs (APTIMA; Hologic, Bedford, MA) in the sentinel lymph nodes of CC patients laparoscopically operated. Design: Prospective pilot study. Setting: The study was conducted in the Department of Advanced Operative and Oncologic Gynecology, Asklepios Hospital, Hamburg, Germany. Patients: 54 women with HPV-positive CC submitted to laparoscopic sentinel node biopsy alone or sentinel node biopsy followed by systematic pelvic and/or para-aortic endoscopic lymphadenectomy. Interventions: All removed sentinel lymph nodes (SLNs) underwent sample collection by cytobrush for the APTIMA assay before frozen section. Measurements: Results obtained with the HPV mRNA test were compared with the definitive histopathological analysis of the SLNs and additional lymph nodes removed. Results: A total of 125 SLNs (119 pelvic and 6 paraaortic) were excised with a mean number of 2.3 SLNs per patient. Final histopathologic analysis confirmed nodal metastases in 10 SLNs from 10 diffeient patients (18%). All the histologically confirmed metastatic lymph nodes were also HPV E6/E7 mRNA positive, resulting in a sensitivity of 100%. Four histologically free sentinel nodes were positive for HPV E6/E7 mRNA, resulting in a specificity of 96.4%. Conclusion: The HPV E6/E7 mRNA assay in the SLNs of patients with CC is feasible and highly accurate. The detection of HPV mRNA in 4 women with negative SLNs might denote a shift from microscopic identification of metastasis to the molecular level. The prognostic value of this findings awaits further verification. (C) 2016 Published by Elsevier Inc.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
| Creators: |
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| URN: | urn:nbn:de:hbz:38-270223 | ||||||||||||||||||||||||||||||||||||
| DOI: | 10.1016/j.jmig.2016.03.003 | ||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | J. Mimim. Invasive Gynecol. | ||||||||||||||||||||||||||||||||||||
| Volume: | 23 | ||||||||||||||||||||||||||||||||||||
| Number: | 5 | ||||||||||||||||||||||||||||||||||||
| Page Range: | S. 748 - 753 | ||||||||||||||||||||||||||||||||||||
| Date: | 2016 | ||||||||||||||||||||||||||||||||||||
| Publisher: | ELSEVIER SCIENCE INC | ||||||||||||||||||||||||||||||||||||
| Place of Publication: | NEW YORK | ||||||||||||||||||||||||||||||||||||
| ISSN: | 1553-4669 | ||||||||||||||||||||||||||||||||||||
| Language: | English | ||||||||||||||||||||||||||||||||||||
| Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||
| Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||
| Subjects: | no entry | ||||||||||||||||||||||||||||||||||||
| Uncontrolled Keywords: |
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| Refereed: | Yes | ||||||||||||||||||||||||||||||||||||
| URI: | http://kups.ub.uni-koeln.de/id/eprint/27022 |
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