Universität zu Köln

Koehler, Sybille, Tellkamp, Frederik ORCID: 0000-0002-0473-7320, Niessen, Carien M., Bloch, Wilhelm, Kerjaschki, Dontscho, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Brinkkoetter, Paul T. (2016). Par3A is dispensable for the function of the glomerular filtration barrier of the kidney. Am. J. Physiol.-Renal Physiol., 311 (1). S. F112 - 8. BETHESDA: AMER PHYSIOLOGICAL SOC. ISSN 1522-1466

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Abstract

Polarity signaling through the atypical PKC (aPKC)-Par polarity complex is essential for the development and maintenance of the podocyte architecture and the function of the glomerular filtration barrier of the kidney. To study the contribution of Par3A in this complex, we generated a novel Pard3 podocyte-specific knockout mouse model by targeting exon 6 of the Pard3 gene. Genetic deletion of Pard3a did not impair renal function, neither at birth nor later in life. Even challenging the animals did not result in glomerular disease. Despite its well-established role in aPKC-mediated signaling, Par3A appears to be dispensable for the function of the glomerular filtration barrier. Moreover, its homolog Pard3b, and not Pard3a, is the dominant Par3 gene expressed in podocytes and found at the basis of the slit diaphragm, where it partially colocalizes with podocin. In conclusion, Par3A function is either dispensable for slit diaphragm integrity, or compensatory mechanisms and a high redundancy of the different polarity proteins, including Par3B, Lgl, or PALS1, maintain the function of the glomerular filtration barrier, even in the absence of Par3A.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koehler, Sybille UNSPECIFIED UNSPECIFIED UNSPECIFIED Tellkamp, Frederik UNSPECIFIED orcid.org/0000-0002-0473-7320 UNSPECIFIED Niessen, Carien M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Kerjaschki, Dontscho UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Brinkkoetter, Paul T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-270453
DOI:	10.1152/ajprenal.00171.2016
Journal or Publication Title:	Am. J. Physiol.-Renal Physiol.
Volume:	311
Number:	1
Page Range:	S. F112 - 8
Date:	2016
Publisher:	AMER PHYSIOLOGICAL SOC
Place of Publication:	BETHESDA
ISSN:	1522-1466
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADHESION MOLECULE JAM; CELL POLARITY; CAENORHABDITIS-ELEGANS; APKC-LAMBDA/IOTA; EPITHELIAL-CELLS; SLIT DIAPHRAGMS; TIGHT JUNCTIONS; PROTEIN PAR-3; PODOCYTE; COMPLEX Multiple languages Physiology; Urology & Nephrology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27045