Faden, Frederik ORCID: 0000-0002-8588-3142, Ramezani, Thomas ORCID: 0000-0003-4681-7844, Mielke, Stefan, Almudi, Isabel ORCID: 0000-0001-7978-4394, Nairz, Knud, Froehlich, Marceli S., Hoeckendorff, Joerg, Brandt, Wolfgang, Hoehenwarter, Wolfgang ORCID: 0000-0002-7669-7524, Dohmen, R. Juergen ORCID: 0000-0002-5756-6780, Schnittger, Arp ORCID: 0000-0001-7067-0091 and Dissmeyer, Nico (2016). Phenotypes on demand via switchable target protein degradation in multicellular organisms. Nat. Commun., 7. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversible and switch-like tuning of protein levels under physiological conditions in vivo. Thereby, developmental effects can be triggered and phenotypes on demand generated. The lt-degron was established to produce conditional and cell-type-specific phenotypes and is generally applicable in a wide range of organisms, from eukaryotic microorganisms to plants and poikilothermic animals. We have successfully applied this system to control the abundance and function of transcription factors and different enzymes by tunable protein accumulation.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Faden, FrederikUNSPECIFIEDorcid.org/0000-0002-8588-3142UNSPECIFIED
Ramezani, ThomasUNSPECIFIEDorcid.org/0000-0003-4681-7844UNSPECIFIED
Mielke, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Almudi, IsabelUNSPECIFIEDorcid.org/0000-0001-7978-4394UNSPECIFIED
Nairz, KnudUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Froehlich, Marceli S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoeckendorff, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Brandt, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoehenwarter, WolfgangUNSPECIFIEDorcid.org/0000-0002-7669-7524UNSPECIFIED
Dohmen, R. JuergenUNSPECIFIEDorcid.org/0000-0002-5756-6780UNSPECIFIED
Schnittger, ArpUNSPECIFIEDorcid.org/0000-0001-7067-0091UNSPECIFIED
Dissmeyer, NicoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-270535
DOI: 10.1038/ncomms12202
Journal or Publication Title: Nat. Commun.
Volume: 7
Date: 2016
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Faculty of Mathematics and Natural Sciences
Divisions: Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
END RULE PATHWAY; HEAT-INDUCIBLE DEGRON; ARABIDOPSIS-THALIANA; IN-VIVO; BETA-GLUCURONIDASE; HIGH-TEMPERATURE; DROSOPHILA-MELANOGASTER; CYTOSOLIC PROTEINS; DNA-REPLICATION; MESSENGER-RNAMultiple languages
Multidisciplinary SciencesMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/27053

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