Universität zu Köln

Schonauer, Anna, Paese, Christian L. B., Hilbrant, Maarten, Leite, Daniel J., Schwager, Evelyn E., Feitosa, Natalia Martins, Eibner, Cornelius ORCID: 0000-0002-8823-8878, Damen, Wim G. M. and McGregor, Alistair P. (2016). The Wnt and Delta-Notch signalling pathways interact to direct pair-rule gene expression via caudal during segment addition in the spider Parasteatoda tepidariorum. Development, 143 (13). S. 2455 - 2464. CAMBRIDGE: COMPANY OF BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

In short-germ arthropods, posterior segments are added sequentially from a segment addition zone (SAZ) during embryogenesis. Studies in spiders such as Parasteatoda tepidariorum have provided insights into the gene regulatory network (GRN) underlying segment addition, and revealed that Wnt8 is required for dynamic Delta (Dl) expression associated with the formation of new segments. However, it remains unclear how these pathways interact during SAZ formation and segment addition. Here, we show that Delta-Notch signalling is required for Wnt8 expression in posterior SAZ cells, but represses the expression of this Wnt gene in anterior SAZ cells. We also found that these two signalling pathways are required for the expression of the spider orthologues of even-skipped (eve) and runt-1 (run-1), at least in part via caudal (cad). Moreover, it appears that dynamic expression of eve in this spider does not require a feedback loop with run-1, as is found in the pair-rule circuit of the beetle Tribolium. Taken together, our results suggest that the development of posterior segments in Parasteatoda is directed by dynamic interactions between Wnt8 and Delta-Notch signalling that are read out by cad, which is necessary but probably not sufficient to regulate the expression of eve and run-1. Our study therefore provides new insights towards better understanding the evolution and developmental regulation of segmentation in other arthropods, including insects.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schonauer, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Paese, Christian L. B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hilbrant, Maarten UNSPECIFIED UNSPECIFIED UNSPECIFIED Leite, Daniel J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwager, Evelyn E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Feitosa, Natalia Martins UNSPECIFIED UNSPECIFIED UNSPECIFIED Eibner, Cornelius UNSPECIFIED orcid.org/0000-0002-8823-8878 UNSPECIFIED Damen, Wim G. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED McGregor, Alistair P. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-271385
DOI:	10.1242/dev.131656
Journal or Publication Title:	Development
Volume:	143
Number:	13
Page Range:	S. 2455 - 2464
Date:	2016
Publisher:	COMPANY OF BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CENTIPEDE STRIGAMIA-MARITIMA; CRICKET GRYLLUS-BIMACULATUS; GROUP-III GENES; CUPIENNIUS-SALEI; SHORT-GERM; TRIBOLIUM-CASTANEUM; ACHAEARANEA-TEPIDARIORUM; PERIPLANETA-AMERICANA; GROWTH-ZONE; DROSOPHILA Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27138