Universität zu Köln

Geiss, Andrea, Sobottke, Rolf, Delank, Karl Stefan and Eysel, Peer (2016). Plasmacytoid dendritic cells and memory T cells infiltrate true sequestrations stronger than subligamentous sequestrations: evidence from flow cytometric analysis of disc infiltrates. Eur. Spine J., 25 (5). S. 1417 - 1428. NEW YORK: SPRINGER. ISSN 1432-0932

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Abstract

Purpose Herniated nucleus pulposus has been considered to induce an adaptive immune response. Antigen recognition by antigen-presenting-cells (APCs) represents an important step within manifestation of an adaptive immune response. Macrophages have been assumed to function as APC, while importance of plasmacytoid dendritic cells for initiation of an immune response directed towards herniated nucleus pulposus has never been examined. The aim of the present study was to assess importance of plasmacytoid dendritic cells for initiation of immune response directed towards herniated discs. Methods Fifteen patients with true sequestrations and three patients with subligamentous sequestrations underwent surgery after their neurological examinations. Disc material was harvested, weighted and digested for 90 min. Separated single cells were counted, stained for plasmacytoid dendritic cells (CD123(+) CD4(+)), macrophages (CD14(+) CD11c(+)) and memory T cells (CD4(+) CD45RO(+)) and analysed by flow cytometry. Both patient groups were compared in cell proportions. Furthermore, patients with true sequestrations (TRUE patients) were subdivided into subgroups based on severity of muscle weakness and results in straight leg raising (SLR) test. Subgroups were compared in cell proportions. Results Plasmacytoid dendritic cells and memory T cells infiltrated true sequestrations stronger than the subligamentous sequestration and plasmacytoid dendritic cells predominated over macrophages in true sequestrations. Highest proportions of plasmacytoid dendritic cells were detected in infiltrates of patients having true sequestrations, severe muscle weakness and negative result in SLR test. Conclusions The findings of the present study indicate that plasmacytoid dendritic cells are involved in initiation of an immune response directed towards herniated nucleus pulposus, while macrophages may reinforce the manifested immune response and mediate disc resorption.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Geiss, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Sobottke, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Delank, Karl Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Eysel, Peer UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-277201
DOI:	10.1007/s00586-015-4325-z
Journal or Publication Title:	Eur. Spine J.
Volume:	25
Number:	5
Page Range:	S. 1417 - 1428
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0932
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN INTERVERTEBRAL DISC; NERVE ROOT COMPRESSION; HERNIATED LUMBAR DISC; NUCLEUS-PULPOSUS; AUTOIMMUNE PROPERTIES; CYTOKINE EXPRESSION; INFLAMMATORY CELLS; INTERFERON; INJURY; TISSUE Multiple languages Clinical Neurology; Orthopedics Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/27720