Universität zu Köln

Roy, Rajika, Haase, Tobias, Ma, Nan ORCID: 0000-0003-4006-1003, Bader, Andreas, Becker, Matthias, Seifert, Martina, Choi, Yeong-Hoon, Falk, Volkmar ORCID: 0000-0002-7911-8620 and Stamm, Christof (2016). Decellularized amniotic membrane attenuates postinfarct left ventricular remodeling. J. Surg. Res., 200 (2). S. 409 - 420. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-8673

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Abstract

Background: Placenta and amnion have been suggested as sources of juvenile cells and tissues for use in surgical regenerative medicine. We previously determined the impact of amniotic epithelial cells induced to undergo epithelial-to-mesenchymal transition (EMT) on myocardial remodeling processes and now evaluated the effects of naive and processed amniotic membrane (AM) on postischemic left ventricular (LV) geometry and function. Methods: Human AM was used in unmodified form (AM), after EMT induction by transforming growth factor beta (EMT-AM), and after decellularization (Decell-AM). After characterization by histology, electron microscopy, splenocyte proliferation assay, and cytokine release, myocardial infarction was induced in 6-8-week old male BALB/c mice by permanent left anterior descending coronary occlusion, and AM patches were sutured to the anterior LV surface (n = 10 per group). Infarcted hearts without AM or sham-operated mice were used as controls (n = 10 each). After 4 weeks, LV pressure-volume curves were recorded using a conductance catheter before the animals were sacrificed and the hearts analyzed by histology. Results: TGF-beta treatment induced EMT-like changes in amniotic epithelial cells but increased AM xenoreactivity in vitro (splenocyte proliferation) and in vivo (CD4+ cell invasion). Moreover, in vitro interleukin-6 release from AM and from cardiac fibroblasts co-incubated with AM was 300- or 100-fold higher than that of interleukin-10, whereas Decell-AM did not release any cytokines. AM-and Decell-AM-treated hearts had smaller infarct size and greater infarct scar thickness than infarct control hearts, but there was no difference in myocardial capillary density or the number of TUNEL positive apoptotic cells. LV contractile function was better in the AM and EMT-AM groups than in infarcted control hearts, but dP/dt max, dP/dt min, stroke work, and cardiac output were best preserved in mice treated with Decell-AM. Volume-based parameters (LV end-systolic and end-diastolic volume as well as LV ejection fraction) did not differ between AM and Decell-AM. Conclusions: Decellularized AM supports postinfarct ventricular dynamics independent of the actual regeneration processes. As a cell-free approach to support the infarcted heart, this concept warrants further investigation. (C) 2016 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Roy, Rajika UNSPECIFIED UNSPECIFIED UNSPECIFIED Haase, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Ma, Nan UNSPECIFIED orcid.org/0000-0003-4006-1003 UNSPECIFIED Bader, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED Falk, Volkmar UNSPECIFIED orcid.org/0000-0002-7911-8620 UNSPECIFIED Stamm, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-286852
DOI:	10.1016/j.jss.2015.08.022
Journal or Publication Title:	J. Surg. Res.
Volume:	200
Number:	2
Page Range:	S. 409 - 420
Date:	2016
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-8673
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESENCHYMAL STEM-CELLS; GROWTH-FACTOR-BETA; EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; MYOCARDIAL-INFARCTION; ALLOGRAFT-REJECTION; HEART-FAILURE; SCAFFOLD; TRANSPLANTATION; REPAIR Multiple languages Surgery Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28685