Universität zu Köln

Rost, S., Akyuez, N., Martinovic, T., Huckhagel, T., Jakovcevski, I. and Schachner, M. (2016). GERMLINE ABLATION OF DERMATAN-4O-SULFOTRANSFERASE1 REDUCES REGENERATION AFTER MOUSE SPINAL CORD INJURY. Neuroscience, 312. S. 74 - 86. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-7544

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Abstract

Chondroitin/dermatan sulfate proteoglycans (CSPGs/DSPGs) are major components of the extracellular matrix. Their expression is generally upregulated after injuries to the adult mammalian central nervous system, which is known for its low ability to restore function after injury. Several studies support the view that CSPGs inhibit regeneration after injury, whereas the functions of DSPGs in injury paradigms are less certain. To characterize the functions of DSPGs in the presence of CSPGs, we studied young adult dermatan-4O-sulfotransferase1-deficient (Chst14(-/-)) mice, which express chondroitin sulfates (CSs), but not dermatan sulfates (DSs), to characterize the functional outcome after severe compression injury of the spinal cord. In comparison to their wild-type (Chst14(+/+)) littermates, regeneration was reduced in Chst14(-/-) mice. No differences between genotypes were seen in the size of spinal cords, numbers of microglia and astrocytes neither in intact nor injured spinal cords after injury. Monoaminergic innervation and re-innervation of the spinal cord caudal to the lesion site as well as expression levels of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were similar in both genotypes, independent of whether they were injured and examined 6 weeks after injury or not injured. These results suggest that, in contrast to CSPGs, DSPGs, being the products of Chst14 enzymatic activity, promote regeneration after injury of the adult mouse central nervous system. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rost, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Akyuez, N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Martinovic, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huckhagel, T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakovcevski, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schachner, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-287263
DOI:	10.1016/j.neuroscience.2015.11.013
Journal or Publication Title:	Neuroscience
Volume:	312
Page Range:	S. 74 - 86
Date:	2016
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-7544
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHONDROITIN SULFATE PROTEOGLYCAN; CENTRAL-NERVOUS-SYSTEM; PROMOTES FUNCTIONAL RECOVERY; ADHESION MOLECULE L1; AXONAL REGENERATION; DERMATAN SULFATE; EXTRACELLULAR-MATRIX; IDURONIC ACID; CLOSE HOMOLOG; TENASCIN-C Multiple languages Neurosciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28726