Weber, Lea V., Al-Refae, Klaudia, Woelk, Gerhard, Bonatz, Gabriele, Altmueller, Janine, Becker, Christian, Gisselmann, Guenter and Hatt, Hanns (2016). Expression and functionality of TRPV1 in breast cancer cells. Breast Cancer-Targets Ther., 8. S. 243 - 253. ALBANY: DOVE MEDICAL PRESS LTD. ISSN 1179-1314

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Abstract

Transient receptor potential (TRP) channels contribute to the regulation of intracellular calcium, which can promote cancer hallmarks in cases of dysregulation of gene transcription and calcium-dependent pro-proliferative or anti-apoptotic mechanisms. Several studies have begun to elucidate the roles of TRPV1, TRPV6, TRPM8, and TRPC1 in cancer progression; however, no study has examined the expression profiles of human TRP channels in breast cancer on a large scale. This study focused on the expression and functionality of TRPV1, a nonselective cation channel that was found to be expressed in different carcinoma tissues. Next-generation sequencing analyses revealed the expression of TRPV1 in several native breast cancer tissues, which was subsequently validated via reverse transcriptase-polymerase chain reaction. Activation of TRPV1 by its ligand capsaicin was associated with the growth inhibition of some cancer cell types; however, the signaling components involved are complex. In this study, stimulation by the TRPV1 agonist, capsaicin, of SUM149PT cells, a model system for the most aggressive breast cancer subtype, triple-negative breast cancer, led to intracellular calcium signals that were diminished by the specific TRPV1 antagonist, capsazepin. Activation of TRPV1 by capsaicin caused significant inhibition of cancer cell growth and induced apoptosis and necrosis. In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Weber, Lea V.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Al-Refae, KlaudiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Woelk, GerhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bonatz, GabrieleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gisselmann, GuenterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hatt, HannsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-288497
DOI: 10.2147/BCTT.S121610
Journal or Publication Title: Breast Cancer-Targets Ther.
Volume: 8
Page Range: S. 243 - 253
Date: 2016
Publisher: DOVE MEDICAL PRESS LTD
Place of Publication: ALBANY
ISSN: 1179-1314
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CAPSAICIN-INDUCED APOPTOSIS; GENE-EXPRESSION; ION CHANNELS; RECEPTOR; CARCINOMA; IDENTIFICATION; PIRARUBICIN; TARGETS; GENOME; TRPM7Multiple languages
OncologyMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/28849

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