Universität zu Köln

Wike, Candice L., Graves, Hillary K., Wason, Arpit, Hawkins, Reva, Gopalakrishnan, Jay, Schumacher, Jill and Tyler, Jessica K. (2016). Excess free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans. Cell Cycle, 15 (16). S. 2216 - 2226. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1551-4005

Full text not available from this repository.

Abstract

The cell tightly controls histone protein levels in order to achieve proper packaging of the genome into chromatin, while avoiding the deleterious consequences of excess free histones. Our accompanying study has shown that a histone modification that loosens the intrinsic structure of the nucleosome, phosphorylation of histone H3 on threonine 118 (H3 T118ph), exists on centromeres and chromosome arms during mitosis. Here, we show that H3 T118ph localizes to centrosomes in humans, flies, and worms during all stages of mitosis. H3 abundance at the centrosome increased upon proteasome inhibition, suggesting that excess free histone H3 localizes to centrosomes for degradation during mitosis. In agreement, we find ubiquitinated H3 specifically during mitosis and within purified centrosomes. These results suggest that targeting of histone H3 to the centrosome for proteasome-mediated degradation is a novel pathway for controlling histone supply, specifically during mitosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wike, Candice L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Graves, Hillary K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wason, Arpit UNSPECIFIED UNSPECIFIED UNSPECIFIED Hawkins, Reva UNSPECIFIED UNSPECIFIED UNSPECIFIED Gopalakrishnan, Jay UNSPECIFIED UNSPECIFIED UNSPECIFIED Schumacher, Jill UNSPECIFIED UNSPECIFIED UNSPECIFIED Tyler, Jessica K. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-289227
DOI:	10.1080/15384101.2016.1192728
Journal or Publication Title:	Cell Cycle
Volume:	15
Number:	16
Page Range:	S. 2216 - 2226
Date:	2016
Publisher:	TAYLOR & FRANCIS INC
Place of Publication:	PHILADELPHIA
ISSN:	1551-4005
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CENP-A; PHOSPHORYLATION; CHROMATIN; UBIQUITYLATION; CHAPERONES; SYSTEM Multiple languages Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/28922