Universität zu Köln

Luo, Xueying, Zhong, Binlong, Hong, Xian, Cui, Yurong, Gao, Ying, Yin, Mengmeng, Tang, Ming, Hescheler, Jurgen and Xi, Jiaoya (2016). Puerarin Exerts a Delayed Inhibitory Effect on the Proliferation of Cardiomyocytes Derived from Murine ES Cells via Slowing Progression through G2/M Phase. Cell. Physiol. Biochem., 38 (4). S. 1333 - 1343. BASEL: KARGER. ISSN 1421-9778

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Abstract

Objective: Puerarin, which shows beneficial and protective effects on cardiovascular diseases, is the main isoflavone extracted from Pueraria lobata (kudzu) root. The aim of this study was to investigate the effects of puerarin on in vitro myocardial proliferation and its underlying mechanism. Methods: Myocardial differentiation of transgenic embryonic stem (ES) cells was performed by embryoid body based differentiation method. The proliferation assay of cardiomyocytes (CMs) derived from ES cells (ES-[Ms) was performed by EdU (5-Ethynyl-2'-deoxyuridine) staining. Flow cytometry was employed to determine the cell cycle distribution and apoptosis of purified ES-CMs. Quantitative real-time PCR was utilized to study the transcription of genes related to cell cycle progression. Signaling pathways relating to proliferation were studied by western blot analysis and application of specific inhibitors. Results: Puerarin exerted a delayed inhibitory effect on the proliferation of ES-CMs at the early-stage differentiation. Meanwhile, puerarin slowed progression through G2/M phase without inducing apoptosis of ES [Ms. Further assays showed that puerarin up-regulated the transcription of Cyclin A2, Cyclin B1 and Cdk1 in ES CMs. The ERK1/2 specific inhibitor PD0325901 and the PI3K specific inhibitor Wortmannin successfully reversed puerarin-induced up-regulation of Cdk1 but not Cyclin A2 and B1. Conclusion: These findings suggest that puerarin inhibits CM proliferation via slowing progression through G2/M phase during early-stage differentiation. (C) 2016 The Author(s) Published by S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Luo, Xueying UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhong, Binlong UNSPECIFIED UNSPECIFIED UNSPECIFIED Hong, Xian UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Yurong UNSPECIFIED UNSPECIFIED UNSPECIFIED Gao, Ying UNSPECIFIED UNSPECIFIED UNSPECIFIED Yin, Mengmeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Jiaoya UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-290016
DOI:	10.1159/000443077
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	38
Number:	4
Page Range:	S. 1333 - 1343
Date:	2016
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIFFERENTIATION; EXPRESSION; APOPTOSIS; PROMOTES; INJURY; ROLES; RAT; B1 Multiple languages Cell Biology; Physiology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29001