Universität zu Köln

Kamper, Matthias, Hamann, Nina, Prein, Carina, Clausen-Schaumann, Hauke ORCID: 0000-0002-9413-0310, Farkas, Zsuzsanna, Aszodi, Attila, Niehoff, Anja, Paulsson, Mats and Zaucke, Frank (2016). Early changes in morphology, bone mineral density and matrix composition of vertebrae lead to disc degeneration in aged collagen IX -/- mice. Matrix Biol., 49. S. 132 - 144. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1569-1802

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Abstract

Collagen IX (Col IX) is an important component of the cartilage extracellular matrix and has been associated with degenerative cartilage disorders and chondrodysplasias in humans. Further, polymorphisms in Col IX are known risk factors for the development of early intervertebral disc (IVD) degeneration. To understand the role of Col IX in the pathogenesis of IVD disorders, the spine of newborn and older Col IX deficient mice was systematically analyzed and compared to C57BL/6N controls. Morphology and bone parameters of the spine from newborn, 6 and 10 months old animals were investigated using mu CT measurements. Histological staining was used to evaluate tissue structure and degree of degeneration. Localization and expression of extracellular matrix proteins was analyzed in depth by immunofluorescence staining, immunoblotting, RT-PCR and in situ hybridization. High resolution imaging and stiffness measurements were performed by atomic force microscopy (AFM). Vertebral bodies of newborn Col IX-deficient mice were smaller and showed an increased mineral density compared to wild type animals. At birth, lack of Col IX led to a disrupted cellular organization in the cartilaginous endplate and a smaller nucleus pulposus of the IVD. Expression levels and localization of other extracellular matrix proteins were strongly altered accompanied by a softening of cartilaginous tissues. In older animals, absence of Col IX caused earlier and more pronounced disc degeneration with annular fissures. The absence of Col IX induces early developmental, structural and biomechanical alterations in both vertebral body and intervertebral disc which eventually cause severe degenerative changes in the aging spine. (C) 2015 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kamper, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamann, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Prein, Carina UNSPECIFIED UNSPECIFIED UNSPECIFIED Clausen-Schaumann, Hauke UNSPECIFIED orcid.org/0000-0002-9413-0310 UNSPECIFIED Farkas, Zsuzsanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Aszodi, Attila UNSPECIFIED UNSPECIFIED UNSPECIFIED Niehoff, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Paulsson, Mats UNSPECIFIED UNSPECIFIED UNSPECIFIED Zaucke, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-290554
DOI:	10.1016/j.matbio.2015.09.005
Journal or Publication Title:	Matrix Biol.
Volume:	49
Page Range:	S. 132 - 144
Date:	2016
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1569-1802
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX; INTERVERTEBRAL DISC; END-PLATE; CARTILAGE; PROTEIN; MOUSE; MATRILIN-3; ABSENCE; GROWTH; GENES Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29055