Weber, Stefanie, Jung, Susan and Doerfler, Walter (2016). DNA methylation and transcription in HERV (K, W, E) and LINE sequences remain unchanged upon foreign DNA insertions. Epigenomics, 8 (2). S. 157 - 166. LONDON: FUTURE MEDICINE LTD. ISSN 1750-192X

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Abstract

Aim: DNA methylation and transcriptional profiles were determined in the regulatory sequences of the human endogenous retroviral (HERV-K, -W, -E) and LINE-1.2 elements and were compared between non-transgenomic and plasmid-transgenomic cells. Methods: DNA methylation profiles in the HERV (K, W, E) and LINE sequences were determined by bisulfite genomic sequencing. The transcription of these genome segments was assessed by quantitative real-time PCR. Results: In HERV-K, HERV-W and LINE-1.2 the levels of DNA methylation ranged between 75 and 98%, while in HERV-E they were around 60%. Nevertheless, the HERV and LINE-1.2 sequences were actively transcribed. No differences were found in comparisons of HERV and LINE-1.2 CpG methylation and transcription patterns between non-transgenomic and plasmid-transgenomic HCT116 cells. Conclusion: The insertion of a 5.6 kbp plasmid into the HCT116 genome had no effect on the HERV and LINE-1.2 methylation and transcription profiles, although other parts of the HCT116 genome had shown marked changes. These repetitive sequences are transcribed, probably because the large number of HERV and LINE-1.2 elements harbor copies with non-or hypo-methylated long terminal repeat sequences.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Weber, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jung, SusanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doerfler, WalterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-291082
DOI: 10.2217/epi.15.109
Journal or Publication Title: Epigenomics
Volume: 8
Number: 2
Page Range: S. 157 - 166
Date: 2016
Publisher: FUTURE MEDICINE LTD
Place of Publication: LONDON
ISSN: 1750-192X
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ENDOGENOUS RETROVIRUSES; CELLS; GENOME; ADENOVIRUS-TYPE-12; INTEGRATION; EXPRESSION; ELEMENTS; GENES; RNAMultiple languages
Genetics & HeredityMultiple languages
Refereed: Yes
URI: http://kups.ub.uni-koeln.de/id/eprint/29108

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