Universität zu Köln

Schaub, F., Simons, H. G., Roters, S., Heindl, L. M., Kugler, W., Bachmann, B. O. and Cursiefen, C. (2016). Influence of 20 % sulfur hexafluoride (SF6) on human corneal endothelial cells. An in vitro study. Ophthalmologe, 113 (1). S. 52 - 58. NEW YORK: SPRINGER. ISSN 1433-0423

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Abstract

Background. In posterior lamellar keratoplasties, such as Descemet membrane endothelial keratoplasty (DMEK) and Descemet's stripping automated endothelial keratoplasty (DSAEK) an air bubble is left inside the anterior chamber to promote graft attachment during the early postoperative period. In the case of insufficient graft adhesion a renewed intracameral air injection is often necessary. The use of sulfur hexafluoride diluted with air (SF6 20 %) as an alternative to pure air may further enhance graft attachment and reduce the rebubbling rate. The effect of SF6 20 % on corneal endothelium is currently unclear and was therefore examined in vitro. Material and methods. For this study 12 human corneoscleral discs were mounted in artificial anterior chambers, the systems were continuously filled with culture medium and the anterior chambers with air (n = 5) or SF6 20 % (n = 7) as tamponade. After 6 days of storage in the incubator endothelial cell density, toxicity on endothelial cells and corneal thickness were evaluated. Results. There were no significant differences in endothelial cell loss (p = 1.000), endothelial cell count (p = 0.648), toxicity on endothelial cells (p = 0.048) and central corneal thickness (p = 0.905) between the two groups after 1 week. The level of significance was defined as p = 0.05 and adjusted to p = 0.0056 according to the Bonferroni correction for multiple testing. Conclusion. The use of SF6 20 % as tamponade in the anterior chamber for posterior lamellar keratoplasty can be proposed as a safe alternative to pure air filling related to endothelial cell loss. Increased toxic effects on the corneal endothelium by SF6 20 % were not detected in this study; however, further prospective clinical trials are needed to examine the long-term effects in humans.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schaub, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Simons, H. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roters, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, L. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kugler, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachmann, B. O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-291368
DOI:	10.1007/s00347-015-0051-5
Journal or Publication Title:	Ophthalmologe
Volume:	113
Number:	1
Page Range:	S. 52 - 58
Date:	2016
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1433-0423
Language:	German
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PARS-PLANA VITRECTOMY; KERATOPLASTY; AIR; DMEK; PERFLUOROPROPANE; GASES; EYES Multiple languages Ophthalmology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/29136