Ma, Shoucai, Lapin, Dmitry, Liu, Li, Sun, Yue, Song, Wen, Zhang, Xiaoxiao, Logemann, Elke, Yu, Dongli, Wang, Jia, Jirschitzka, Jan, Han, Zhifu, Schulze-Lefert, Paul, Parker, Jane E. and Chai, Jijie (2020). Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme. Science, 370 (6521). S. 1184 - 1195. WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 1095-9203

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Abstract

Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The Hyaloperonospora arabidopsidis effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of Arabidopsis NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/ Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ma, ShoucaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lapin, DmitryUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liu, LiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sun, YueUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Song, WenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhang, XiaoxiaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Logemann, ElkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yu, DongliUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wang, JiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jirschitzka, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Han, ZhifuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulze-Lefert, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parker, Jane E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-308659
DOI: 10.1126/science.abe3069
Journal or Publication Title: Science
Volume: 370
Number: 6521
Page Range: S. 1184 - 1195
Date: 2020
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 1095-9203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/30865

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