Ilyas, Shaista, Ullah, Nighat K., Ilyas, Muhammad, Wennhold, Kerstin, Iqbal, Maria, Schloesser, Hans A., Hussain, Muhammad S. and Mathur, Sanjay (2020). Mediating the Fate of Cancer Cell Uptake: Dual-Targeted Magnetic Nanovectors with Biotin and Folate Surface Ligands. ACS Biomater. Sci. Eng., 6 (11). S. 6138 - 6148. WASHINGTON: AMER CHEMICAL SOC. ISSN 2373-9878

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Abstract

Recognition of folate and biotin surface receptors by dual-functionalized nanoparticles (NPs) is key for site-selective receptor-mediated transport of anticancer drugs to cancer cells. We present here dopamine-capped iron oxide nanoprobes (Fe3O4, 10 +/- 2 nm) containing two surface-grafted biologically relevant ligands, namely, folic acid (FA) and biotin (BT). The covalent attachment of both FA and BT on Fe3O4 nanoparticles was achieved by following carbodiimide coupling and click-chemistry protocols. The dual-function Fe 3 0 4 probes were delivered into E-G7 and human HeLa cancer cell lines and tested toward their cellular uptake by immunofluorescence and flow cytometry analysis. Owing to receptor-mediated endocytosis, enhanced accumulation of nanoprobes in cancer cells was successfully monitored by confocal laser microscopy. When compared to dual-function probes, single-functionalized nanoparticles possessing either FA or BT ligands showed significantly reduced uptake in the tested cell lines, underlining the superior interaction potential of dual-purpose probes. A time-dependent receptor-mediated endocytosis of FA-Fe3O4-BT nanovectors was demonstrated by flow cytometry analysis, whereas the unfunctionalized NPs did not show any specificity in terms of uptake. Besides their specific uptake, the surface-functionalized nanoparticles exhibited promising cytotoxicity profiles by demonstrating good viability of more than 95% with analogous cancer cell lines. Our results demonstrate that dual and/or multivariate conjugation of receptor-specific ligands on NPs is highly effective in molecular recognition of surface biomarkers that enhances their potential in anticancer treatment for pretargeting-radio strategies based on biotin/avidin interactions.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Ilyas, ShaistaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ullah, Nighat K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ilyas, MuhammadUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wennhold, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Iqbal, MariaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schloesser, Hans A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hussain, Muhammad S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mathur, SanjayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-313127
DOI: 10.1021/acsbiomaterials.0c00771
Journal or Publication Title: ACS Biomater. Sci. Eng.
Volume: 6
Number: 11
Page Range: S. 6138 - 6148
Date: 2020
Publisher: AMER CHEMICAL SOC
Place of Publication: WASHINGTON
ISSN: 2373-9878
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
Materials Science, BiomaterialsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/31312

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