Universität zu Köln

Hartmann, Fabian Stefan Franz, Clermont, Lina, Antelmann, Haike and Seibold, Gerd Michael (2020). The Industrial Organism Corynebacterium glutamicum Requires Mycothiol as Antioxidant to Resist Against Oxidative Stress in Bioreactor Cultivations. Antioxidants, 9 (10). BASEL: MDPI. ISSN 2076-3921

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Abstract

In aerobic environments, bacteria are exposed to reactive oxygen species (ROS). To avoid an excess of ROS, microorganisms are equipped with powerful enzymatic and non-enzymatic antioxidants. Corynebacterium glutamicum, a widely used industrial platform organism, uses mycothiol (MSH) as major low molecular weight (LMW) thiol and non-enzymatic antioxidant. In aerobic bioreactor cultivations, C. glutamicum becomes exposed to oxygen concentrations surpassing the air saturation, which are supposed to constitute a challenge for the intracellular MSH redox balance. In this study, the role of MSH was investigated at different oxygen levels (pO(2)) in bioreactor cultivations in C. glutamicum. Despite the presence of other highly efficient antioxidant systems, such as catalase, the MSH deficient Delta mshC mutant was impaired in growth in bioreactor experiments performed at pO(2) values of 30%. At a pO(2) level of 20%, this growth defect was abolished, indicating a high susceptibility of the MSH-deficient mutant towards elevated oxygen concentrations. Bioreactor experiments with C. glutamicum expressing the Mrx1-roGFP2 redox biosensor revealed a strong oxidative shift in the MSH redox potential (E-MSH) at pO(2) values above 20%. This indicates that the LMW thiol MSH is an essential antioxidant to maintain the robustness and industrial performance of C. glutamicum during aerobic fermentation processes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hartmann, Fabian Stefan Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED Clermont, Lina UNSPECIFIED UNSPECIFIED UNSPECIFIED Antelmann, Haike UNSPECIFIED UNSPECIFIED UNSPECIFIED Seibold, Gerd Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-316345
DOI:	10.3390/antiox9100969
Journal or Publication Title:	Antioxidants
Volume:	9
Number:	10
Date:	2020
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-3921
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language METHIONINE SULFOXIDE REDUCTASE; HYDROGEN-PEROXIDE; DYNAMIC CHANGES; REDOX SWITCHES; THIOL; OXYGEN; SUPEROXIDE; ACID; BIOSYNTHESIS; MYCOREDOXIN Multiple languages Biochemistry & Molecular Biology; Chemistry, Medicinal; Food Science & Technology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31634