Tellkamp, Frederik ORCID: 0000-0002-0473-7320, Lang, Franziska, Ibanez, Alejandro ORCID: 0000-0001-7479-8623, Abraham, Lena, Quezada, Galo, Guenther, Stefan, Looso, Mario, Tann, Fabian Jannik, Mueller, Daniela, Cemic, Franz, Hemberger, Juergen, Steinfartz, Sebastian and Krueger, Marcus (2020). Proteomics of Galapagos Marine Iguanas Links Function of Femoral Gland Proteins to the Immune System. Mol. Cell. Proteomics, 19 (9). ROCKVILLE: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1535-9484

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Abstract

Femoral glands secrete a wax-like substance on the inner side of lizard hind legs, which is thought to function as a mode of chemical communication. Though the minor volatile fraction is well studied, the major protein fraction remains enigmatic. Here, we use proteomics to analyze proteins in femoral gland secretions of the Galapagos marine iguana. Although we found no evidence for proteins and peptides involved in chemical communication, we found several immune-regulatory proteins which also demonstrate anti-microbial functions. Accordingly, we show that femoral gland proteins and peptides function as a barrier against microbial infection and may prevent the rapid degradation of volatile substances. Communication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of the substances involved for most systems. The femoral gland is an organ that secretes a waxy substance involved in chemical communication in lizards. Although the lipids and volatile substances secreted by the femoral glands have been investigated in several biochemical studies, the protein composition and functions of secretions remain completely unknown. Applying a proteomic approach, we provide the first attempt to comprehensively characterize the protein composition of femoral gland secretions from the Galapagos marine iguana. Using samples from several organs, the marine iguana proteome was assembled by next-generation sequencing and MS, resulting in 7513 proteins. Of these, 4305 proteins were present in the femoral gland, including keratins, small serum proteins, and fatty acid-binding proteins. Surprisingly, no proteins with discernible roles in partner recognition or inter-species communication could be identified. However, we did find several proteins with direct associations to the innate immune system, including lysozyme C, antileukoproteinase (ALP), pulmonary surfactant protein (SFTPD), and galectin (LGALS1) suggesting that the femoral glands function as an important barrier to infection. Furthermore, we report several novel anti-microbial peptides from the femoral glands that show similar action againstEscherichia coliandBacillus subtilissuch as oncocin, a peptide known for its effectiveness against Gram-negative pathogens. This proteomics data set is a valuable resource for future functional protein analysis and demonstrates that femoral gland secretions also perform functions of the innate immune system.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Tellkamp, FrederikUNSPECIFIEDorcid.org/0000-0002-0473-7320UNSPECIFIED
Lang, FranziskaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ibanez, AlejandroUNSPECIFIEDorcid.org/0000-0001-7479-8623UNSPECIFIED
Abraham, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Quezada, GaloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guenther, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Looso, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Tann, Fabian JannikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mueller, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cemic, FranzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hemberger, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Steinfartz, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Krueger, MarcusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-321422
DOI: 10.1074/mcp.RA120.001947
Journal or Publication Title: Mol. Cell. Proteomics
Volume: 19
Number: 9
Date: 2020
Publisher: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication: ROCKVILLE
ISSN: 1535-9484
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EVOLUTIONARY ANALYSIS; PREDICTION; LIZARD; IDENTIFICATION; RECOGNITION; MORPHOLOGY; PEPTIDES; PLATFORM; DATABASE; TOOLMultiple languages
Biochemical Research MethodsMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32142

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