Koehler, Anna, Morgelin, Matthias, Gebauer, Jan M., Oecal, Sinan, Imhof, Thomas, Koch, Manuel, Nagata, Kazuhiro, Paulsson, Mats, Zaucke, Frank, Baumann, Ulrich and Sengle, Gerhard (2020). New specific HSP47 functions in collagen subfamily chaperoning. Faseb J., 34 (9). S. 12040 - 12053. HOBOKEN: WILEY. ISSN 1530-6860

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Abstract

Although collagens are the most abundant proteins implicated in various disease pathways, essential mechanisms required for their proper folding and assembly are poorly understood. Heat-shock protein 47 (HSP47), an ER-resident chaperone, was mainly reported to fulfill key functions in folding and secretion of fibrillar collagens by stabilizing pro-collagen triple-helices. In this study, we demonstrate unique functions of HSP47 for different collagen subfamilies. Our results show that HSP47 binds to the N-terminal region of procollagen I and is essential for its secretion. However, HSP47 ablation does not majorly impact collagen VI secretion, but its lateral assembly. Moreover, specific ablation ofHsp47in murine keratinocytes revealed a new role for the transmembrane collagen XVII triple-helix formation. Incompletely folded collagen XVII C-termini protruding from isolated HSP47 null keratinocyte membrane vesicles could be fully restored upon the application of recombinant HSP47. Thus, our study expands the current view regarding the client repertoire and function of HSP47, as well as emphasizes its importance for transmembrane collagen folding.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Koehler, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morgelin, MatthiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gebauer, Jan M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Oecal, SinanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Imhof, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, ManuelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagata, KazuhiroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Paulsson, MatsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zaucke, FrankUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Baumann, UlrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sengle, GerhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-325653
DOI: 10.1096/fj.202000570R
Journal or Publication Title: Faseb J.
Volume: 34
Number: 9
Page Range: S. 12040 - 12053
Date: 2020
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1530-6860
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MOLECULAR CHAPERONE; TRIPLE-HELIX; ENDOPLASMIC-RETICULUM; RECOGNITION; PROTEIN; CARTILAGE; ACID; PH; MICROFIBRILS; EXPRESSIONMultiple languages
Biochemistry & Molecular Biology; Biology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32565

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