Fabianska, Izabela, Pesch, Lina, Koebke, Eva, Gerlach, Nina and Bucher, Marcel (2020). Neighboring plants divergently modulate effects of loss-of-function in maize mycorrhizal phosphate uptake on host physiology and root fungal microbiota. PLoS One, 15 (6). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Maize, a main crop worldwide, establishes a mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi providing nutrients to the roots from soil volumes which are normally not in reach of the non-colonized root. The mycorrhizal phosphate uptake pathway (MPU) spans from extraradical hyphae to root cortex cells housing fungal arbuscules and promotes the supply of phosphate to the mycorrhizal host in exchange for photosynthetic carbon. This symbiotic association with the mycobiont has been shown to affect plant host nutritional status and growth performance. However, whether and how the MPU affects the root microbial community associated with mycorrhizal hosts in association with neighboring plants, remains to be demonstrated. Here the maize germinal Mu transposon insertion mutantpht1;6, defective in mycorrhiza-specific Pi transporterPHT1;6gene, and wild type B73 (wt) plants were grown in mono- and mixed culture and examined under greenhouse and field conditions. Disruption of the MPU inpht1;6resulted in strongly diminished growth performance, in reduced P allocation to photosynthetic source leaves, and in imbalances in leaf elemental composition beyond P. At the microbial community level a loss of MPU activity had a minor effect on the root-associated fungal microbiome which was almost fully restricted to AM fungi of the Glomeromycotina. Moreover, while wt grew better in presence ofpht1;6,pht1;6accumulated little biomass irrespective of whether it was grown in mono- or mixed culture and despite of an enhanced fungal colonization of its roots in co-culture with wt. This suggested that a functional MPU is prerequisite to maintain maize growth and that neighboring plants competed for AM fungal Pi in low P soil. Thus future strategies towards improving yield in maize populations on soils with low inputs of P fertilizer could be realized by enhancing MPU at the individual plant level while leaving the root-associated fungal community largely unaffected.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Fabianska, IzabelaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pesch, LinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koebke, EvaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gerlach, NinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bucher, MarcelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-329755
DOI: 10.1371/journal.pone.0232633
Journal or Publication Title: PLoS One
Volume: 15
Number: 6
Date: 2020
Publisher: PUBLIC LIBRARY SCIENCE
Place of Publication: SAN FRANCISCO
ISSN: 1932-6203
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TRANSPORTER; GENE; DIVERSITY; PATHWAY; GROWTH; SOIL; COLONIZATION; ABUNDANCE; RESPONSES; NITROGENMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/32975

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