Albrecht, Benjamin, Voronina, Elena, Schipke, Carola, Peters, Oliver, Parr, Maria Kristina ORCID: 0000-0001-7407-8300, Diaz-Hernandez, M. Dolores and Schloerer, Nils E. (2020). Pursuing Experimental Reproducibility: An Efficient Protocol for the Preparation of Cerebrospinal Fluid Samples for NMR-Based Metabolomics and Analysis of Sample Degradation. Metabolites, 10 (6). BASEL: MDPI. ISSN 2218-1989

Full text not available from this repository.

Abstract

NMR-based metabolomics investigations of human biofluids offer great potential to uncover new biomarkers. In contrast to protocols for sample collection and biobanking, procedures for sample preparation prior to NMR measurements are still heterogeneous, thus compromising the comparability of the resulting data. Herein, we present results of an investigation of the handling of cerebrospinal fluid (CSF) samples for NMR metabolomics research. Origins of commonly observed problems when conducting NMR experiments on this type of sample are addressed, and suitable experimental conditions in terms of sample preparation and pH control are discussed. Sample stability was assessed by monitoring the degradation of CSF samples by NMR, hereby identifying metabolite candidates, which are potentially affected by sample storage. A protocol was devised yielding consistent spectroscopic data as well as achieving overall sample stability for robust analysis. We present easy to adopt standard operating procedures with the aim to establish a shared sample handling strategy that facilitates and promotes inter-laboratory comparison, and the analysis of sample degradation provides new insights into sample stability.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Albrecht, BenjaminUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Voronina, ElenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schipke, CarolaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Peters, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Parr, Maria KristinaUNSPECIFIEDorcid.org/0000-0001-7407-8300UNSPECIFIED
Diaz-Hernandez, M. DoloresUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schloerer, Nils E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-331200
DOI: 10.3390/metabo10060251
Journal or Publication Title: Metabolites
Volume: 10
Number: 6
Date: 2020
Publisher: MDPI
Place of Publication: BASEL
ISSN: 2218-1989
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PATTERN-RECOGNITION; BLOOD-PLASMA; CSF; STABILITY; DISEASE; SERUM; IDENTIFICATION; SPECTROSCOPY; METABOLITES; MENINGITISMultiple languages
Biochemistry & Molecular BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33120

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item