Stanicek, Laura, Lozano-Vidal, Noelia ORCID: 0000-0003-4115-0227, Bink, Diewertje Ilse, Hooglugt, Aukie, Yao, Wenjie, Wittig, Ilka, van Rijssel, Jos ORCID: 0000-0001-8077-1371, van Buul, Jaap Diederik, van Bergen, Anke ORCID: 0000-0002-1843-5657, Klems, Alina, Ramms, Anne Sophie, Le Noble, Ferdinand, Hofmann, Patrick, Szulcek, Robert ORCID: 0000-0003-0450-7338, Wang, ShengPeng, Offermanns, Stefan, Ercanoglu, Meryem Seda, Kwon, Hyouk-Bum, Stainier, Didier, Huveneers, Stephan ORCID: 0000-0002-1091-475X, Kurian, Leo ORCID: 0000-0001-7466-5169, Dimmeler, Stefanie and Boon, Reinier Abraham (2020). Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function. Commun. Biol., 3 (1). NEW YORK: NATURE PUBLISHING GROUP. ISSN 2399-3642

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Abstract

Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are stabilized by the shear stress-induced long non-coding RNA LASSIE (linc00520). Silencing of LASSIE in endothelial cells impairs cell survival, cell-cell contacts and cell alignment in the direction of flow. LASSIE associates with junction proteins (e.g. PECAM-1) and the intermediate filament protein nestin, as identified by RNA affinity purification. The AJs component VE-cadherin showed decreased stabilization, due to reduced interaction with nestin and the microtubule cytoskeleton in the absence of LASSIE. This study identifies LASSIE as link between nestin and VE-cadherin, and describes nestin as crucial component in the endothelial response to shear stress. Furthermore, this study indicates that LASSIE regulates barrier function by connecting AJs to the cytoskeleton. Stanicek et al identify a shear stress-induced long non-coding RNA they name LASSIE, which stabilises junctions between endothelial cells through interactions with junctional and cytoskeletal proteins. This study provides insights into how a transcript that does not encode a protein controls endothelial response to forces associated with blood flow and endothelial barrier function.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Stanicek, LauraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lozano-Vidal, NoeliaUNSPECIFIEDorcid.org/0000-0003-4115-0227UNSPECIFIED
Bink, Diewertje IlseUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hooglugt, AukieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yao, WenjieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wittig, IlkaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Rijssel, JosUNSPECIFIEDorcid.org/0000-0001-8077-1371UNSPECIFIED
van Buul, Jaap DiederikUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
van Bergen, AnkeUNSPECIFIEDorcid.org/0000-0002-1843-5657UNSPECIFIED
Klems, AlinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ramms, Anne SophieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Le Noble, FerdinandUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hofmann, PatrickUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Szulcek, RobertUNSPECIFIEDorcid.org/0000-0003-0450-7338UNSPECIFIED
Wang, ShengPengUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Offermanns, StefanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ercanoglu, Meryem SedaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kwon, Hyouk-BumUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stainier, DidierUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huveneers, StephanUNSPECIFIEDorcid.org/0000-0002-1091-475XUNSPECIFIED
Kurian, LeoUNSPECIFIEDorcid.org/0000-0001-7466-5169UNSPECIFIED
Dimmeler, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Boon, Reinier AbrahamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-332888
DOI: 10.1038/s42003-020-0987-0
Journal or Publication Title: Commun. Biol.
Volume: 3
Number: 1
Date: 2020
Publisher: NATURE PUBLISHING GROUP
Place of Publication: NEW YORK
ISSN: 2399-3642
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
VE-CADHERIN; ADHERENS JUNCTION; CELL JUNCTIONS; ADHESION; IDENTIFICATION; CYTOSKELETON; ORGANIZATION; INTEGRATION; SIZEMultiple languages
Biology; Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/33288

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