Universität zu Köln

Daverkausen-Fischer, Lea, Motyl-Eisemann, Myriam, Draga, Margarethe, Scaal, Martin and Proels, Felicitas (2020). Protein expression pattern of the molecular chaperone Mdg1/ERdj4 during embryonic development. Histochem. Cell Biol., 154 (3). S. 255 - 264. NEW YORK: SPRINGER. ISSN 1432-119X

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Abstract

The vertebrate-specific co-chaperone Mdg1/ERdj4, which is localized in the endoplasmic reticulum, controls the folding and degradation of proteins. We characterized its protein pattern during chick embryonic development. During early development, Mdg1/ERdj4 protein is present in mesenchymal and epithelial cells. In mesenchymal cells, it has a salt and pepper pattern. In contrast, during epithelial tissue differentiation, Mdg1/ERdj4 marks the basal and/or apical compartment of epithelial linings. The distinct protein pattern in epithelial tissue might point to its role in organizing and maintaining the epithelial structure. This could be achieved, e.g. by controlling folding and secretion of membrane-bound receptors or by inhibiting the IRE1 alpha-Xbp1s-SNAI1/2-induced mesenchymalization. High Mdg1/ERdj4 protein levels are maintained in tissue with sustained secretory activity as in ependymal cells or enterocytes, substantiating its important role for secretion. We conclude that the transient elevation of Mdg1/ERdj4 protein levels controls the differentiation of epithelial linings while constitutive high levels are closely linked to secretory activity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Daverkausen-Fischer, Lea UNSPECIFIED UNSPECIFIED UNSPECIFIED Motyl-Eisemann, Myriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Draga, Margarethe UNSPECIFIED UNSPECIFIED UNSPECIFIED Scaal, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Proels, Felicitas UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-334273
DOI:	10.1007/s00418-020-01881-x
Journal or Publication Title:	Histochem. Cell Biol.
Volume:	154
Number:	3
Page Range:	S. 255 - 264
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-119X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language B MEMBER 9; DNAJ HOMOLOG; CO-CHAPERONE; IN-VITRO; FAMILY; HSP70; BIP; COCHAPERONE; ERDJ4; GENE Multiple languages Cell Biology; Microscopy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/33427